| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,856,434 | A→G | S85P (TCA→CCA) | msrB ← | peptide‑methionine (R)‑S‑oxide reductase MsrB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,856,434 | 0 | A | G | 94.4% | 56.6 / ‑3.1 | 18 | S85P (TCA→CCA) | msrB | peptide‑methionine (R)‑S‑oxide reductase MsrB |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (10/7); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
TAAAGAGGCAGAGTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACG > NZ_CP009273/1856315‑1856569 | tAAAGAGGCAGAGTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGAGCTGCATTCCATGTGGCAAGTCTTTGATATAACg > 2:208030/1‑138 (MQ=255) agGCAGAGTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGa < 2:580999/139‑1 (MQ=255) aaCACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACt > 1:104771/1‑138 (MQ=255) aTAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGt > 1:677726/1‑139 (MQ=255) cGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCa < 1:173761/80‑1 (MQ=255) cGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCa > 2:173761/1‑80 (MQ=255) cGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGgccagcca > 1:465499/1‑139 (MQ=255) ccGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGc < 2:388869/139‑1 (MQ=255) aTGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTg > 1:707395/1‑139 (MQ=255) gATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTggg > 1:98644/1‑138 (MQ=255) acaGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAg < 1:153103/138‑1 (MQ=255) acaGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAg < 1:208030/138‑1 (MQ=255) cGAATTTCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGa < 2:98644/139‑1 (MQ=255) tCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCaaa < 1:352989/138‑1 (MQ=255) tCTATGCGCTGCATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCaaa < 2:707395/138‑1 (MQ=255) cATTCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACg > 2:854972/1‑139 (MQ=255) tCCATGTGGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCg > 2:96893/1‑139 (MQ=255) gtgGCAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACg > 2:275009/1‑139 (MQ=255) | TAAAGAGGCAGAGTTAACACAATAACGTTCGCCCGTTGGCTGCGGCCCGTCGGGGAAGACATGCCCCAGATGGGCATCACAGTTACCGCAACGAATTTCTATGCGCTGCATTCCATGTGACAAGTCTTTGATATAACGAATGGATTCTTCACTTACCGGTTCGTAGAAACTGGGCCAGCCACAGCCGGAATCATACTTGGTTTGGGAATGAAACAGCGGGGCATCGCAGATCAAACAGTGATATACGCCGTCACG > NZ_CP009273/1856315‑1856569 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |