| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,868,665 | T→C | S19S (AGT→AGC) | yeaL → | DUF441 domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,868,665 | 0 | T | C | 77.3% | 39.9 / 10.7 | 22 | S19S (AGT→AGC) | yeaL | DUF441 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base C (8/9); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
AAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCTTTTTTCCGTGGATTGAAAAACAGGGGCTAAGTATCGGGATAATCATTCTGACGATTGGCGTTATG > NZ_CP009273/1868530‑1868797 | aaaCGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCAt < 1:235502/139‑1 (MQ=255) cTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGc < 2:617373/138‑1 (MQ=255) tccttcctGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGCCGttt > 1:595981/1‑138 (MQ=255) tcctGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAAtt > 1:733791/1‑139 (MQ=255) gcaAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGCCGTTTCAATTCTGgtgt < 1:828224/139‑1 (MQ=255) aGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGt < 1:691612/139‑1 (MQ=255) aGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGCCGTTTCAATTCt > 1:537888/1‑120 (MQ=255) aGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGCCGTTTCAATTCt < 2:537888/120‑1 (MQ=255) ggCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTca < 1:724720/139‑1 (MQ=255) attattTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCacac < 1:599958/137‑1 (MQ=255) cACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCTTTTTTCCGTg > 1:177325/1‑138 (MQ=255) cTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTca < 2:777683/96‑1 (MQ=255) cTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTca > 1:777683/1‑96 (MQ=255) cTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCTTTTTTCCGTGGATTGAAAAACAGGGGc > 1:182387/1‑138 (MQ=255) cTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTca < 1:61405/91‑1 (MQ=255) cTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTca > 2:61405/1‑91 (MQ=255) gCTCGGGTTAGCTGCGCTGGGCTTTATCAGCCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCTTTTTTCCGTGGATTGAAAAACAGGGGCTAAGTATCGGGAt > 1:609309/1‑138 (MQ=255) gTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCTTTTTTCCGTGGATTGAAAAACAGGGGCTAAGTATCGGGATAATCAtt < 2:733791/139‑1 (MQ=255) ggCTTTATCAGCCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCTTTTTTCCGTGGATTGAAAAACAGGGGCTAAGTATCGGGATAATCATTCTGACGATTgg < 2:182387/137‑1 (MQ=255) tATCAGTCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCTTTTTTCCg < 2:554749/82‑1 (MQ=255) tATCAGTCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCTTTTTTCCg > 1:554749/1‑82 (MQ=255) tATCAGCCATAACACCACTGTCGCCGTTTCAATTCTGGAGTTAAACATTGTCCGCGTCACACCGTTAAGCACATTTTTTCCGTGGCTTGAAAAACAGGGGCTAAGTATAGGGATAATCATTCTGACGATTGGCGTTATg > 2:337767/1‑139 (MQ=255) | AAACGTAAAGATTTTCTACTTCCTTCCTGCAGCAAGCGTAAAGTAAGCAGGCTTATTATTTTTTGGCAAGGAAACCACGATGTTTGATGTCACTTTGCTGATCCTGCTCGGGTTAGCTGCGCTGGGCTTTATCAGTCATAACACCACTGTCGCCGTTTCAATTCTGGTGTTAATCATTGTCCGCGTCACACCGTTAAGCACCTTTTTTCCGTGGATTGAAAAACAGGGGCTAAGTATCGGGATAATCATTCTGACGATTGGCGTTATG > NZ_CP009273/1868530‑1868797 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |