| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,879,784 | T→G | V288G (GTG→GGG) | yeaW → | carnitine monooxygenase subunit YeaW |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,879,784 | 0 | T | G | 90.3% | 96.1 / 3.6 | 31 | V288G (GTG→GGG) | yeaW | carnitine monooxygenase subunit YeaW |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base G (15/13); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
TCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGTTCCGTCCGGAAGATTTACGTCTGGTTGAAAGCGTA > NZ_CP009273/1879657‑1879917 | tCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGaaa < 2:452779/139‑1 (MQ=255) aTTTGAAG‑GGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACg < 1:109812/139‑1 (MQ=255) ggATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCAc > 1:414074/1‑138 (MQ=255) gCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCAc > 2:399387/1‑134 (MQ=255) gCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCAc < 1:399387/134‑1 (MQ=255) cGGCATTCCACGGTTTCTGGCTGTTGCCGTGCACGATGCTGAACGTCCCCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATg < 1:172883/138‑1 (MQ=255) cGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATg > 2:799197/1‑138 (MQ=255) cGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATg < 1:528217/138‑1 (MQ=255) cGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATg > 1:95067/1‑138 (MQ=255) tCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGt > 1:29451/1‑138 (MQ=255) ggTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACg < 1:649237/109‑1 (MQ=255) ggTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACg > 2:649237/1‑109 (MQ=255) gTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACcga < 1:799197/139‑1 (MQ=255) ggCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGaaa > 1:223029/1‑84 (MQ=255) ggCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGaaa < 2:223029/84‑1 (MQ=255) cGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATGTTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGa < 2:717727/139‑1 (MQ=255) gATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAg < 2:414074/139‑1 (MQ=255) cACCCCGATCAAAGGGATGATGACGGTCATTTCTGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGAt > 1:747821/1‑139 (MQ=255) cACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGAt > 2:691100/1‑139 (MQ=255) cACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGAt > 1:166452/1‑139 (MQ=255) cACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGAt > 1:256295/1‑139 (MQ=255) cGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGtt < 2:400603/139‑1 (MQ=255) aTCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGTtcc > 1:677106/1‑139 (MQ=255) tgatgaCGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAg > 2:232627/1‑110 (MQ=255) tgatgaCGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAg < 1:232627/110‑1 (MQ=255) tgatgaCGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGTTCCGTCCgg > 1:699385/1‑135 (MQ=255) atgaCGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGTTCCGTCCGGAAGAtt < 2:95067/139‑1 (MQ=255) ggTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGTTCCGTCCGGAAGATTTACGt < 2:256295/139‑1 (MQ=255) aaTTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGTTCCGTCCGGAAGATTTACGTCTGGTTGaaa < 2:61106/138‑1 (MQ=255) ccGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGTTCCGTCCGGAAGATTTACGTCTGGTTGAAAGCGTa > 2:522616/1‑138 (MQ=255) ccGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGTTCCGTCCGGAAGATTTACGTCTGGTTGAAAGCGTa > 1:472695/1‑138 (MQ=255) | TCCGAACAGTCGTTTAAATTTGAAGAGGGTACGGATGCGGCATTCCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGTGGTATCGCGATGTGTTCCGTCCGGAAGATTTACGTCTGGTTGAAAGCGTA > NZ_CP009273/1879657‑1879917 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |