Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,932,990	A→G	E362G (GAA→GGA)	pyk →	pyruvate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,932,990	0	A	G	100.0%	83.1 / NA	24	E362G (GAA→GGA)	pyk	pyruvate kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/12);  total (12/12)

TCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCAT  >  NZ_CP009273/1932857‑1933125                                                                                                                                      |                                                                                                                                        tCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGgagaag                                                                                                                                    >  2:664482/1‑139 (MQ=255)   tGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGc                                                                                                                                   >  1:191821/1‑138 (MQ=255)          aCTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCa                                                                                                                           <  2:822251/139‑1 (MQ=255)             gCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGt                                                                                                                        >  2:451255/1‑139 (MQ=255)              ccGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGt                                                                                                                        <  1:330926/138‑1 (MQ=255)                    ggCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCa                                                                                                                   <  2:272304/137‑1 (MQ=255)                    ggCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCa                                                                                                                   <  2:725686/137‑1 (MQ=255)                       aGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTa                                                                                                              <  1:102861/139‑1 (MQ=255)                                       cGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTg                                                                                              <  1:118947/139‑1 (MQ=255)                                                         cgTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGatc                                                                            <  1:367077/139‑1 (MQ=255)                                                         cgTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGatc                                                                            <  1:79186/139‑1 (MQ=255)                                                                                                            tCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGaa                                                             >  2:635971/1‑103 (MQ=255)                                                                                                            tCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGaa                                                             <  1:635971/103‑1 (MQ=255)                                                                                                            tCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGc                         >  1:784960/1‑139 (MQ=255)                                                                                                            tCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGc                         >  2:345758/1‑139 (MQ=255)                                                                                                            tCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGc                         >  1:584471/1‑139 (MQ=255)                                                                                                                    ttCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACcg                                                <  2:260352/108‑1 (MQ=255)                                                                                                                    ttCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACcg                                                >  1:260352/1‑108 (MQ=255)                                                                                                                    ttCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCt                 >  2:777405/1‑139 (MQ=255)                                                                                                                      cAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGatc                                                                            <  2:819580/78‑1 (MQ=255)                                                                                                                      cAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGatc                                                                            >  1:819580/1‑78 (MQ=255)                                                                                                                      cAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCggg                                                        <  2:830269/98‑1 (MQ=255)                                                                                                                      cAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCggg                                                        >  1:830269/1‑98 (MQ=255)                                                                                                                       aGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGc               >  2:580283/1‑138 (MQ=255)                                                                                                                                   tgGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCAt  >  1:378580/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                        TCTGCAGAAACTGCCGCTGGGCAGTATCCGTCAGAAACCGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACCGCGCTGATGACCTCCCGTATCAGCTCTGGTCTGCCAATTTTCGCCAT  >  NZ_CP009273/1932857‑1933125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑