Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,955,016 A→G D233G (GAC→GGC)  argS → arginine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,955,0160AG94.4% 113.1 / ‑0.2 36D233G (GAC→GGC) argSarginine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (16/18);  total (17/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

ACCTTGAAGGTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGATGCTGCCAGGAATTGTGGCGGATCTCAAAGCCAAAGGTCTGG  >  NZ_CP009273/1954881‑1955150
                                                                                                                                       |                                                                                                                                      
aCCTTGAAGGTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCAt                                                                                                                                     <  2:842788/139‑1 (MQ=255)
aCCTTGAAGGTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCAt                                                                                                                                     >  2:183569/1‑139 (MQ=255)
      aaGGTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCa                                                                                                                                <  1:265530/138‑1 (MQ=255)
      aaGGTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCAt                                                                                                                               >  1:735328/1‑139 (MQ=255)
          tttCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACg                                                                                                                           <  1:559528/139‑1 (MQ=255)
            tCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACg                                                                                                                           >  1:120311/1‑137 (MQ=255)
            tCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACg                                                                                                                           >  1:477887/1‑137 (MQ=255)
            tCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACg                                                                                                                           <  2:120311/137‑1 (MQ=255)
            tCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACg                                                                                                                           >  1:841604/1‑137 (MQ=255)
                    gATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGa                                                                                                                 <  2:47517/139‑1 (MQ=255)
                    gATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGa                                                                                                                 <  1:551409/139‑1 (MQ=255)
                          aaaaaGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCAcc                                                                                                            >  1:47919/1‑138 (MQ=255)
                              aGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGa                                                                                                       <  2:477887/139‑1 (MQ=255)
                                           agatgaagaGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTg                                                                                          <  2:100465/139‑1 (MQ=255)
                                                       cGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGa                                                                                                                 >  2:543666/1‑104 (MQ=255)
                                                       cGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGa                                                                                                                 <  1:543666/104‑1 (MQ=255)
                                                       cGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCg                                                                               <  1:863079/138‑1 (MQ=255)
                                                       cGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGt                                                                              >  2:694434/1‑139 (MQ=255)
                                                       cGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGt                                                                              >  1:138180/1‑139 (MQ=255)
                                                          cGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGtgat                                                                           >  2:519793/1‑139 (MQ=255)
                                                               gcgcACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGt                                                                      >  1:718774/1‑139 (MQ=255)
                                                                            cGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGaaa                                                           <  2:47919/137‑1 (MQ=255)
                                                                               ggTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACg                                                                                            <  1:10399/101‑1 (MQ=255)
                                                                               ggTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACg                                                                                            >  2:10399/1‑101 (MQ=255)
                                                                                 tAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAg                                                                                                                        >  1:677194/1‑71 (MQ=255)
                                                                                 tAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAg                                                                                                                        <  2:677194/71‑1 (MQ=255)
                                                                                 tAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCg                                                              >  1:105898/1‑129 (MQ=255)
                                                                                 tAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCg                                                              <  2:105898/129‑1 (MQ=255)
                                                                                 tAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTa                                                    >  1:587042/1‑139 (MQ=255)
                                                                                        gCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGAc                                                                                  <  2:608395/102‑1 (MQ=255)
                                                                                        gCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGAc                                                                                  >  1:608395/1‑102 (MQ=255)
                                                                                           aaGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGAtg                                          <  2:701900/139‑1 (MQ=255)
                                                                                             gCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGAtgct                                        <  2:718774/139‑1 (MQ=255)
                                                                                                gTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGATGCTGcc                                     <  1:183569/139‑1 (MQ=255)
                                                                                                gTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGATGCTGcc                                     <  2:138180/139‑1 (MQ=255)
                                                                                                                                    tCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGATGCTGCCAGGAATTGTGGCGGATCTCAAAGCCAAAGGCCTgg  >  2:429273/1‑138 (MQ=255)
                                                                                                                                       |                                                                                                                                      
ACCTTGAAGGTTTCTACCGCGATGCGAAAAAGCATTACGATGAAGATGAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACAACCCGATGCTGCCAGGAATTGTGGCGGATCTCAAAGCCAAAGGTCTGG  >  NZ_CP009273/1954881‑1955150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑