Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	225,426	T→C	noncoding (12/77 nt)	BW25113_RS01035 →	tRNA‑Asp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	225,426	0	T	C	100.0%	58.4 / NA	17	noncoding (12/77 nt)	BW25113_RS01035	tRNA‑Asp
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

CCAGGCATCAAATTAAGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGTCTGTATATCTATTATTGC  >  NZ_CP009273/225355‑225552                                                                        |                                                                                                                               ccAGGCATCAAATTAAGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACt                                                             >  1:287627/1‑139 (MQ=11)             ttAAGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGcc                                                 <  2:785038/139‑1 (MQ=255)                     tAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTaag                                                      >  1:123310/1‑126 (MQ=37)                     tAAGCCGGTCAGAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTaag                                                      <  2:123310/126‑1 (MQ=255)                          cGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCt                                    >  2:772754/1‑139 (MQ=255)                            gTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTc                                   >  1:210080/1‑138 (MQ=255)                                       ggtggtTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGtttttttt                       >  1:250959/1‑139 (MQ=255)                                           gtTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGt                    <  1:757337/138‑1 (MQ=255)                                           gtTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGt                    <  2:161650/138‑1 (MQ=255)                                           gtTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGt                    <  2:447527/138‑1 (MQ=255)                                           gtTGTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGt                    <  2:67559/138‑1 (MQ=255)                                              gTAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGTCTg                 <  2:250959/138‑1 (MQ=255)                                               tAAAAGAATTCGGTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGTCTGt                <  1:256667/138‑1 (MQ=255)                                                           gTGGAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGTCTGTATATCTATTATTg   >  1:498795/1‑139 (MQ=255)                                                              gAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCg                                  <  1:443589/105‑1 (MQ=255)                                                              gAGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCg                                  >  2:443589/1‑105 (MQ=255)                                                               aGCGGTAGTCCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGCTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGTCTGTATATCTATTATTGc  >  2:285066/1‑136 (MQ=255)                                                                        |                                                                                                                               CCAGGCATCAAATTAAGCAGTAAGCCGGTCATAAAACCGGTGGTTGTAAAAGAATTCGGTGGAGCGGTAGTTCAGTCGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCGTTCCGCCACTTATTAAGAAGCCTCGAGTTAACGCTCGAGGTTTTTTTTCGTCTGTATATCTATTATTGC  >  NZ_CP009273/225355‑225552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑