| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,000,880 | A→G | D415G (GAC→GGC) | amyA → | alpha‑amylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,000,880 | 0 | A | G | 96.3% | 86.7 / ‑6.1 | 27 | D415G (GAC→GGC) | amyA | alpha‑amylase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (16/10); minor base T (1/0); total (17/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TGCGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGACCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCT > NZ_CP009273/2000752‑2001008 | tGCGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAAc > 2:258795/1‑139 (MQ=255)tGCGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAAc > 1:307886/1‑139 (MQ=255)tGCGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAAc > 1:643816/1‑139 (MQ=255) aTTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCAt > 1:91822/1‑139 (MQ=255) ttACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCAtt < 1:258795/139‑1 (MQ=255) tCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCg > 1:77041/1‑139 (MQ=255) cGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCAcc > 1:840582/1‑139 (MQ=255) cAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACcgacg < 1:411253/139‑1 (MQ=255) atagataTGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCt < 1:237242/139‑1 (MQ=255) tagataTGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTg > 2:345491/1‑139 (MQ=255) gCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCgtggtg > 1:136999/1‑139 (MQ=255) ccAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCgtggtg < 1:24705/138‑1 (MQ=255) tgatgaGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGTCCATCCGAACTGCa > 1:181261/1‑74 (MQ=255) tgatgaGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCa < 2:181261/74‑1 (MQ=255) ttAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGc > 1:796264/1‑138 (MQ=255) aTTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGaa < 1:345491/138‑1 (MQ=255) gCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCt > 2:234882/1‑97 (MQ=255) gCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCt < 1:234882/97‑1 (MQ=255) cccGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCAt < 2:840582/139‑1 (MQ=255) ttATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCgg < 1:366313/62‑1 (MQ=255) ttATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCgg > 2:366313/1‑62 (MQ=255) ttATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCt > 1:551423/1‑139 (MQ=255) ttATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCt > 1:659735/1‑139 (MQ=255) ttATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCt > 1:418629/1‑139 (MQ=255) ttATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCt > 2:694447/1‑139 (MQ=255) aTTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCg < 2:196849/79‑1 (MQ=255) aTTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCg > 1:196849/1‑79 (MQ=255) | TGCGCATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGACCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACGGCAATAAAACCT > NZ_CP009273/2000752‑2001008 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |