| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,014,103 | A→C | Y179S (TAC→TCC) | fliM → | flagellar motor switch protein FliM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,014,103 | 0 | A | C | 88.5% | 72.9 / 4.1 | 26 | Y179S (TAC→TCC) | fliM | flagellar motor switch protein FliM |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base C (14/9); total (15/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01 | |||||||||||
GGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATT > NZ_CP009273/2013994‑2014229 | ggTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTa > 2:207854/1‑139 (MQ=255) tCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTAcc < 2:157444/139‑1 (MQ=255) cgAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCaa > 1:592648/1‑138 (MQ=255) ccGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGcc < 1:413933/139‑1 (MQ=255) aaCAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGaa > 1:183739/1‑139 (MQ=255) aaCAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGaa > 2:161774/1‑139 (MQ=255) cgcgTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACa > 2:732672/1‑139 (MQ=255) cgcgTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACa > 2:538349/1‑139 (MQ=255) cgcgTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACa > 1:588821/1‑139 (MQ=255) cgTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACAtt < 1:207854/139‑1 (MQ=255) catcaACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATtgtg > 1:45706/1‑139 (MQ=255) aaaCTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTc > 2:683196/1‑138 (MQ=255) gcgcTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCAtgtg < 2:225132/138‑1 (MQ=255) gcgcTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCAtgtg > 2:384180/1‑138 (MQ=255) gcgcTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCAtgtg > 1:513327/1‑138 (MQ=255) gcgcTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCAtgtg > 1:724166/1‑138 (MQ=255) cgcTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGcc < 1:562507/127‑1 (MQ=255) cgcTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGcc > 2:562507/1‑127 (MQ=255) ccTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACa < 2:292944/90‑1 (MQ=255) ccTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACa > 1:292944/1‑90 (MQ=255) ccTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCg > 1:479324/1‑138 (MQ=255) ccTGGAAGGCGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAAGGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCg < 2:479324/138‑1 (MQ=255) cGATTAATCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAatat < 2:183739/139‑1 (MQ=255) tCCGCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATctgcctg < 2:202028/139‑1 (MQ=255) gCTGGAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCa < 1:550305/139‑1 (MQ=255) gAAGTTGAGTCCGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCAtt < 2:513327/137‑1 (MQ=255) | GGTCGCGAGTTTACCCATACCGAACAGCGCGTCATCAACCGCATGTTGAAACTGGCGCTTGAAGGCTATAGCGACGCCTGGAAGGCGATTAATCCGCTGGAAGTTGAGTACGTGCGTTCGGAAATGCAGGTGAAATTTACCAATATCACCACCTCGCCGAACGACATTGTGGTTAACACGCCGTTCCATGTGGAGATTGGCAACCTGACCGGCGAATTTAATATCTGCCTGCCATT > NZ_CP009273/2013994‑2014229 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |