Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,030,709	A→G	S309P (TCG→CCG)	hprS ←	two‑component system sensor histidine kinase HprS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,030,709	0	A	G	100.0%	68.6 / NA	21	S309P (TCG→CCG)	hprS	two‑component system sensor histidine kinase HprS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/11);  total (10/11)

GATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGA  >  NZ_CP009273/2030573‑2030831                                                                                                                                         |                                                                                                                           gATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGt                                                                                                                          >  1:815323/1‑139 (MQ=255)       aTTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGtt                                                                                                                    >  1:643423/1‑139 (MQ=255)           gTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCAc                                                                                                                <  2:401411/139‑1 (MQ=255)                      aTTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGt                                                                                                     <  2:815323/139‑1 (MQ=255)                                             aTTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAAc                                                                              <  2:643423/139‑1 (MQ=255)                                                  aCTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTAt                                                                         <  1:560754/139‑1 (MQ=255)                                                         cTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGt                                                                           >  1:122399/1‑130 (MQ=255)                                                         cTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGt                                                                           <  2:122399/130‑1 (MQ=255)                                                                                          ttCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATg                                 >  2:2084/1‑139 (MQ=255)                                                                                                  aGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTAt                                                                         <  2:253881/91‑1 (MQ=255)                                                                                                  aGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTAt                                                                         >  1:253881/1‑91 (MQ=255)                                                                                                    tCCAACACATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCCCCAAAACATTGTTTTTATCTGCCCTGGCAAGa                                                                                 <  2:375468/81‑1 (MQ=255)                                                                                                    tCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGa                                                                                 >  1:375468/1‑81 (MQ=255)                                                                                                    tCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCaa                       >  2:731936/1‑139 (MQ=255)                                                                                                    tCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCaa                       >  2:780372/1‑139 (MQ=255)                                                                                                                   aCTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACcgcg                                                        >  1:795292/1‑91 (MQ=255)                                                                                                                   aCTTCCTTATTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACcgcg                                                        <  2:795292/91‑1 (MQ=255)                                                                                                                           aTTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGa  <  2:502616/137‑1 (MQ=255)                                                                                                                           aTTGAGAGAAAGCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGa  <  1:2084/137‑1 (MQ=255)                                                                                                                                      gCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTg             <  1:623634/115‑1 (MQ=255)                                                                                                                                      gCGGGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTg             >  2:623634/1‑115 (MQ=255)                                                                                                                                         |                                                                                                                           GATAACATTCGTTGTAGTAAAATTTTATCCGCAAAGATTTGCTGATTGCACTCGACCTTAAAGCAAATCTCTTTCTCGTCTGAAAGGTATTCAAGATAGTCCAACAAATTTTCGACTTCCTTATTGAGAGAAAGCGAGTCCAGTTTCACCAAAACATTGTTTTTATCTGCCCTGGCAAGAAACAGTATGTTCTCTGTTAACCGCGAAATATTTTCCAGCTCTTCAATGTTTCCGGCAATTGTTTTTTGATATTCAGCGA  >  NZ_CP009273/2030573‑2030831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑