BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,037,050	T→G	intergenic (‑12/‑82)	BW25113_RS10320 ← / → mtfA	tRNA‑Ser/DgsA anti‑repressor MtfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,037,050	0	T	G	100.0%	145.0 / NA	39	intergenic (‑12/‑82)	BW25113_RS10320/mtfA	tRNA‑Ser/DgsA anti‑repressor MtfA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (19/20); total (19/20)

ATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGC  >  NZ_CP009273/2036939‑2037178

aTTGATAAAGTGGCGGAGAGAGGGGGATTTGGACCCCAGGTAGAGGTGCACCTACTCCGGTTTTCGAGAAAGGTCCGTTCAGCCGCTTCGGGACTTATCCGTTCAGACGGTGGTCATGATGCCAGGTAATTTGGCCttt                                                                                                       >  2:124262/1‑139 (MQ=255)

  tGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCAtt                                                                                                        >  2:835186/1‑136 (MQ=255)

  tGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCAtt                                                                                                        <  1:835186/136‑1 (MQ=255)

    aTAAAGTGGCGGAGAGAGGGGGATTTGAAGCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                    <  2:420587/138‑1 (MQ=255)

    aTAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGTCCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                    <  1:211705/138‑1 (MQ=255)

    aTAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                    <  1:688832/138‑1 (MQ=255)

                     gggggATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCaa                                                                                  <  1:384592/139‑1 (MQ=255)

                      ggggATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAAt                                                                                 >  2:561333/1‑139 (MQ=255)

                              gAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCAtt                                                                                                        >  2:90883/1‑108 (MQ=255)

                              gAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCAtt                                                                                                        <  1:90883/108‑1 (MQ=255)

                                    ccGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGAc                                                                   >  2:450318/1‑139 (MQ=255)

                                                         cGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACgat                                               >  2:550969/1‑138 (MQ=255)

                                                           gTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCCGGCAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACgatgat                                            >  1:839062/1‑139 (MQ=255)

                                                           gTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                    >  1:335387/1‑83 (MQ=255)

                                                           gTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                    <  2:335387/83‑1 (MQ=255)

                                                           gTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACgatgat                                            >  2:840048/1‑139 (MQ=255)

                                                              ttCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                    <  1:75896/80‑1 (MQ=255)

                                                              ttCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTaa                                                                                                    >  2:75896/1‑80 (MQ=255)

                                                                cGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAGAACGATGATTAAGt                                       >  1:777094/1‑139 (MQ=255)

                                                                cGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAg                                        <  1:379735/138‑1 (MQ=255)

                                                                        gTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTg                                >  2:753319/1‑138 (MQ=255)

                                                                        gTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTg                                >  2:239714/1‑138 (MQ=255)

                                                                         tCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGa                              <  1:108413/139‑1 (MQ=255)

                                                                         tCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGa                              <  1:685179/139‑1 (MQ=255)

                                                                         tCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGa                              <  1:298060/139‑1 (MQ=255)

                                                                             ttCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAg                           >  2:811085/1‑138 (MQ=255)

                                                                             ttCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAg                           >  1:20923/1‑138 (MQ=255)

                                                                                aGCCGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACa                       <  2:830455/139‑1 (MQ=255)

                                                                                  ccGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGtt                                                                           <  2:272965/85‑1 (MQ=255)

                                                                                  ccGCTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGtt                                                                           >  1:272965/1‑85 (MQ=255)

                                                                                     cTCCGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAAt                  >  1:829415/1‑139 (MQ=255)

                                                                                        cGGCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCa                <  1:450318/138‑1 (MQ=255)

                                                                                         ggCATCTCTCCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGc              <  1:598923/139‑1 (MQ=255)

                                                                                                ctcCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGa                              <  1:777078/116‑1 (MQ=255)

                                                                                                ctcCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGa                              >  2:777078/1‑116 (MQ=255)

                                                                                                 tcCGTTCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCa        <  1:550969/137‑1 (MQ=255)

                                                                                                     ttCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAg               >  2:112709/1‑126 (MQ=255)

                                                                                                     ttCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAg               <  1:112709/126‑1 (MQ=255)

                                                                                                     ttCAGATGGTGGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGc  <  2:20923/139‑1 (MQ=255)

ATTGATAAAGTGGCGGAGAGAGGGGGATTTGAACCCCCGGTAGAGTTGCCCCTACTCCGGTTTTCGAGACCGGTCCGTTCAGCCGCTCCGGCATCTCTCCGTTCAGATGGTTGCCATGATGCCAGGAAATTTGGCATTTTAACAGTCCCTGTCCGTGCAATTTTGTTCAAGTGACGAGTTTGCGAGCAAAACGATGATTAAGTGGCCCTGGAAAGTACAAGAATCAGCACATCAAACTGC  >  NZ_CP009273/2036939‑2037178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`