Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,039,724	A→G	T436A (ACA→GCA)	yeeJ →	inverse autotransporter adhesin YeeJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,039,724	0	A	G	100.0%	154.4 / NA	42	T436A (ACA→GCA)	yeeJ	inverse autotransporter adhesin YeeJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/19);  total (23/19)

CGCAATGCAGAAACAGCTTGACCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGACAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGT  >  NZ_CP009273/2039588‑2039858                                                                                                                                         |                                                                                                                                       cgcAATGCAGAAACAGCTTGACCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCa                                                                                                                                      <  1:39669/139‑1 (MQ=255)          gAAACAGCTTGACCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTg                                                                                                                             <  1:138444/139‑1 (MQ=255)           aaaCAGCTTGACCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGa                                                                                                                            >  1:216611/1‑139 (MQ=255)           aaaCAGCTTGACCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGa                                                                                                                            >  1:229337/1‑139 (MQ=255)           aaaCAGCTTGACCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGa                                                                                                                            >  2:806851/1‑139 (MQ=255)                gCTTGACCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAggg                                                                                                                       <  2:656255/139‑1 (MQ=255)                     aCCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGt                                                                                                                   >  1:283493/1‑138 (MQ=255)                           aTGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGGTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGgaga                                                                                                            <  2:81441/139‑1 (MQ=255)                                           gCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGtt                                                                                            >  1:851697/1‑139 (MQ=255)                                            cGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGttt                                                                                           >  1:497337/1‑139 (MQ=255)                                                     gcaggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTa                                                                                   <  2:554169/138‑1 (MQ=255)                                                      caggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGAcc                                                                                                                                  >  1:500509/1‑90 (MQ=255)                                                      caggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGAcc                                                                                                                                  <  2:500509/90‑1 (MQ=255)                                                      caggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTa                                                                                   <  2:735964/137‑1 (MQ=255)                                                      caggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTa                                                                                   >  1:735964/1‑137 (MQ=255)                                                      caggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTAc                                                                                  >  1:171126/1‑138 (MQ=255)                                                      caggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTAc                                                                                  >  1:841502/1‑138 (MQ=255)                                                      caggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTAc                                                                                  <  2:293265/138‑1 (MQ=255)                                                      caggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTAc                                                                                  >  1:82801/1‑138 (MQ=255)                                                      caggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTAc                                                                                  >  1:374650/1‑138 (MQ=255)                                                                          gtggATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGa                                                                <  2:229337/136‑1 (MQ=255)                                                                          gtggATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAg                                                             >  2:226969/1‑139 (MQ=255)                                                                          gtggATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAg                                                             >  2:482471/1‑139 (MQ=255)                                                                           tggATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAgg                                                            <  2:290701/139‑1 (MQ=255)                                                                               tCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCta                                                         >  2:342627/1‑138 (MQ=255)                                                                                cGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCtat                                                        >  2:694194/1‑138 (MQ=255)                                                                                cGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCtat                                                        >  2:747586/1‑138 (MQ=255)                                                                                 gCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCtata                                                       <  2:283493/138‑1 (MQ=255)                                                                                 gCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCtata                                                       <  1:806851/138‑1 (MQ=255)                                                                                  caacaacaaTATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAAc                                                     >  2:680903/1‑139 (MQ=255)                                                                                       acaaTATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGa                                                <  2:841502/139‑1 (MQ=255)                                                                                             tCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGaa                                                                                                      >  2:464919/1‑79 (MQ=255)                                                                                             tCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGaa                                                                                                      <  1:464919/79‑1 (MQ=255)                                                                                             tCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCAc                                          <  2:764560/139‑1 (MQ=255)                                                                                                  cTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCAc                                     >  2:634693/1‑139 (MQ=255)                                                                                                     gAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACt                                    <  1:680903/137‑1 (MQ=255)                                                                                                          tCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCt                             >  2:478671/1‑139 (MQ=255)                                                                                                           cGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTTACAAGGAAGTCAGAAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACTGCGAAGCCACCGCTCTGGAAGCTg                            >  1:364324/1‑139 (MQ=255)                                                                                                            gCAACAAAGAACTGGTTCGCATGACCCTGGAAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCCCTGGTTTCGTCGCTACAAACCAAATATGACCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGc                           <  2:364324/139‑1 (MQ=255)                                                                                                             cAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAggg                                                                                                                       <  2:680571/46‑1 (MQ=255)                                                                                                             cAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAggg                                                                                                                       >  1:680571/1‑46 (MQ=255)                                                                                                              aaaaaaGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCg                         <  2:497337/139‑1 (MQ=255)                                                                                                                                ctgaccctggcAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACaa       <  2:216611/139‑1 (MQ=255)                                                                                                                                    ccctggcAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCaaa                 <  2:617902/125‑1 (MQ=255)                                                                                                                                    ccctggcAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCaaa                 >  1:617902/1‑125 (MQ=255)                                                                                                                                     cctggcAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGt  <  1:226969/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CGCAATGCAGAAACAGCTTGACCCGAATGAAGTCGCTGCACGGCGTAGCCTTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGACAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAACGTCGAAGCCACCGCACTGGAAGCTGCCGGTGGCAAAGTGGTCACAACGGGT  >  NZ_CP009273/2039588‑2039858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑