Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,066,165	A→G	G382G (GGA→GGG)	agn43 →	autotransporter adhesin Ag43

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,066,165	0	A	G	100.0%	90.4 / NA	26	G382G (GGA→GGG)	agn43	autotransporter adhesin Ag43
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/11);  total (15/11)

AAGCTGATAATGTCGTACTGGAAAATGGCGGACGCCTGGATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCC  >  NZ_CP009273/2066029‑2066300                                                                                                                                         |                                                                                                                                        aaGCTGATAATGTCGTACTGGAAAATGGCGGACGCCTGGATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGgaa                                                                                                                                       <  1:724046/139‑1 (MQ=255)          aTGTCGTACTGGAAAATGGCGGACGCCTGGATGTGCTGACCGGACACACAGCCATTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGgtgt                                                                                                                              <  2:178448/139‑1 (MQ=255)                     gAAAATGGCGGACGCCTGGATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGcc                                                                                                                    >  1:119921/1‑138 (MQ=255)                                     tGGATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGt                                                                                                   >  1:85252/1‑139 (MQ=255)                                       gATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGt                                                                                                   <  2:85252/137‑1 (MQ=255)                                       gATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCa                                                                                                 <  2:119921/139‑1 (MQ=255)                                         tgtgCTGCCCGGACACACAGCCACTAATTCCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCTGTGCCGCTGTCAGt                                                                                               <  1:415163/139‑1 (MQ=255)                                            gCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGt                                                                                                   <  1:303168/132‑1 (MQ=255)                                            gCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGt                                                                                                   >  2:303168/1‑132 (MQ=255)                                             cTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTa                                                                                           >  2:342671/1‑139 (MQ=255)                                                aCCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTAccc                                                                                        >  2:450794/1‑139 (MQ=255)                                                aCCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTAccc                                                                                        >  1:741525/1‑139 (MQ=255)                                                     acacacAGCCACTAATGCCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGc                                                                                   <  1:846194/139‑1 (MQ=255)                                                          caGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCg                                                                                                       <  2:127406/114‑1 (MQ=255)                                                          caGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACAGCTGGCCGATTCCGGTGCCg                                                                                                       >  1:127406/1‑114 (MQ=255)                                                            gCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGa                                                                             >  2:654057/1‑138 (MQ=255)                                                               aCTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATgg                                                                                                                                    <  1:272705/80‑1 (MQ=255)                                                               aCTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATgg                                                                                                                                    >  2:272705/1‑80 (MQ=255)                                                                     aCCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCAtt                                                                     >  2:267763/1‑137 (MQ=255)                                                                       ccGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGGGCGGCGGAAAGGCATTCAg                                                                  >  2:371341/1‑138 (MQ=255)                                                                                         aaCGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCg                                               >  1:483611/1‑139 (MQ=255)                                                                                                  tGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTg                                      <  2:77825/139‑1 (MQ=255)                                                                                                        cAACGGTGGCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTg                                <  1:620446/139‑1 (MQ=255)                                                                                                                gCACCGCCACCACCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAgg                        <  2:741525/139‑1 (MQ=255)                                                                                                                      ccaccaCCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGtt                   >  1:162444/1‑138 (MQ=255)                                                                                                                      ccaccaCCGTATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGtt                   >  1:646037/1‑138 (MQ=255)                                                                                                                               tATCCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCt         >  2:50073/1‑139 (MQ=255)                                                                                                                                      ggggAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGcc  >  1:275015/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                        AAGCTGATAATGTCGTACTGGAAAATGGCGGACGCCTGGATGTGCTGACCGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCAGGCGGATGCCCTGATGCTGGAAAAAGGCAGTTCATTCACGCTGAACGCC  >  NZ_CP009273/2066029‑2066300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑