Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,069,946	A→G	Y53C (TAT→TGT)	yeeS →	RadC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,069,946	0	A	G	100.0%	93.5 / NA	26	Y53C (TAT→TGT)	yeeS	RadC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/16);  total (10/16)

ACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACCACAATGCCGCTGCCGT  >  NZ_CP009273/2069818‑2070066                                                                                                                                 |                                                                                                                         aCGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGaacaac                                                                                                                <  2:33561/139‑1 (MQ=255)                ccTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGAtt                                                                                                 >  2:142149/1‑139 (MQ=255)                                    aaCCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCAc                                                                             <  1:127991/139‑1 (MQ=255)                                      cccGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCg                                                                           <  2:662823/139‑1 (MQ=255)                                      cccGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCg                                                                           <  1:457277/139‑1 (MQ=255)                                         ggCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCa                                                                        >  2:687041/1‑139 (MQ=255)                                               gCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATc                                                                   <  1:436538/138‑1 (MQ=255)                                                     aCCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCa                                                            <  2:403460/139‑1 (MQ=255)                                                                    gcaCGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATcccc                                             >  1:277769/1‑139 (MQ=255)                                                                          gAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAg                                       >  1:92231/1‑139 (MQ=255)                                                                               gCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGc                                                                                               <  2:566272/78‑1 (MQ=255)                                                                               gCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGc                                                                                               >  1:566272/1‑78 (MQ=255)                                                                               gCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACgg                                                         >  1:585431/1‑116 (MQ=255)                                                                               gCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACgg                                                         <  2:585431/116‑1 (MQ=255)                                                                               gCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGAt                                   >  2:773209/1‑138 (MQ=255)                                                                                    ttCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAAcg                             <  1:773209/139‑1 (MQ=255)                                                                                         aaCATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAAcgcg                           <  2:618062/136‑1 (MQ=255)                                                                                         aaCATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGcc                         <  2:51826/138‑1 (MQ=255)                                                                                         aaCATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGcc                         <  2:567893/138‑1 (MQ=255)                                                                                         aaCATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAAAGCcccc                        >  2:189722/1‑139 (MQ=255)                                                                                              ggCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTAc                   >  1:45497/1‑139 (MQ=255)                                                                                                         aGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACCACAATGCCGc        <  2:92231/139‑1 (MQ=255)                                                                                                             tGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAAc                              <  1:672100/113‑1 (MQ=255)                                                                                                             tGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAAc                              >  2:672100/1‑113 (MQ=255)                                                                                                               aagaaTTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACCACAATGCCGCTGCCGt  <  2:493751/139‑1 (MQ=255)                                                                                                               aagaaTTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACCACAATGCCGCTGCCGt  <  2:690582/139‑1 (MQ=255)                                                                                                                              tgtgTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAAcg                             <  2:309504/97‑1 (MQ=255)                                                                                                                              tgtgTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAAcg                             >  1:309504/1‑97 (MQ=255)                                                                                                                                 |                                                                                                                         ACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGATTAAACGCGCCCTGTACCACAATGCCGCTGCCGT  >  NZ_CP009273/2069818‑2070066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑