Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,088,811	T→C	V69A (GTC→GCC)	hisA →	1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,088,811	0	T	C	100.0%	110.9 / NA	32	V69A (GTC→GCC)	hisA	1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

CAGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGGCTCCACCGCGGTGAAATCACAAGATATGGTGAAAGG  >  NZ_CP009273/2088675‑2088946                                                                                                                                         |                                                                                                                                        cAGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCcg                                                                                                                                       <  1:714590/139‑1 (MQ=255)   gcgcGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCcgcg                                                                                                                                     <  1:79980/139‑1 (MQ=255)              gTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGt                                                                                                                          >  2:294207/1‑139 (MQ=255)                 aCGGCCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCGCCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTcc                                                                                                                       <  1:616563/139‑1 (MQ=255)                     cccGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTg                                                                                                                   <  1:294207/139‑1 (MQ=255)                     cccGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTg                                                                                                                   >  2:376149/1‑139 (MQ=255)                      ccGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGc                                                                                                                  <  2:284956/139‑1 (MQ=255)                       cGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTg                                                                                                                   >  1:482671/1‑137 (MQ=255)                       cGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTg                                                                                                                   >  1:843873/1‑137 (MQ=255)                         cTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAgg                                                                                                               >  2:464413/1‑139 (MQ=255)                             cgcgATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTgg                                                                                                           >  1:39226/1‑139 (MQ=255)                                     cAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTggcggcggc                                                                                                    <  1:438276/138‑1 (MQ=255)                                                  cgcAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCgaag                                                                                       >  2:449419/1‑138 (MQ=255)                                                                 tgctgcACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGt                                                                        >  1:769977/1‑138 (MQ=255)                                                                   ctgcACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTAc                                                                     >  1:589389/1‑139 (MQ=255)                                                                      cACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTg                                                                   <  1:399959/138‑1 (MQ=255)                                                                         cTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAg                                                               <  2:39226/139‑1 (MQ=255)                                                                           ggtggATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCg                                                             >  1:455998/1‑139 (MQ=255)                                                                           ggtggATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCg                                                             >  1:527432/1‑139 (MQ=255)                                                                           ggtggATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGCGCCGCTGGGCGGCGGCGTGCGTAGCGGAGAAGACGTGGCGGCGGTAATTGAAGcc                                                             >  2:427050/1‑138 (MQ=255)                                                                                     aCCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTgcg                                                    <  2:15021/138‑1 (MQ=255)                                                                                         gggCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGcgcgcg                                                >  2:656607/1‑138 (MQ=255)                                                                                               aaGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGt                                         <  2:589389/139‑1 (MQ=255)                                                                                                     cGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTggcg                                                                                                         <  2:751999/69‑1 (MQ=255)                                                                                                     cGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTggcg                                                                                                         >  1:751999/1‑69 (MQ=255)                                                                                                                 aaaTCCCGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGGCTCCACCGCGGTGa                        <  2:522881/138‑1 (MQ=255)                                                                                                                      ccGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGGCTCCACCGCGGTGAAATca                   <  2:413343/138‑1 (MQ=255)                                                                                                                       cGCTGATTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGGCTCCACCGCGGTGAAATcaca                 <  1:449419/139‑1 (MQ=255)                                                                                                                            aTTAAAACCCTGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGGCTCCACCGCGGTGAAATCACAAGata            <  1:305166/139‑1 (MQ=255)                                                                                                                                      tGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTgcg                                                    >  1:627666/1‑89 (MQ=255)                                                                                                                                      tGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTgcg                                                    <  2:627666/89‑1 (MQ=255)                                                                                                                                      tGGCCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGGTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGGCTCCACCGCGGTGAAATCACAAGATATGGTGAAAgg  >  2:319879/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                        CAGCGCGATTACGGTAACGACCCGCTGCCGCGATTGCAGGATTACGCCGCGCAGGGTGCCGAAGTGCTGCACCTGGTGGATCTGACCGGGGCAAAAGATCCGGCTAAACGTCAAATCCCGCTGATTAAAACCCTGGTCGCGGGCGTTAACGTTCCGGTGCAGGTTGGCGGCGGCGTGCGTAGCGAAGAAGATGTGGCGGCGTTACTGGAAGCGGGCGTTGCGCGCGTAGTGGTCGGCTCCACCGCGGTGAAATCACAAGATATGGTGAAAGG  >  NZ_CP009273/2088675‑2088946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑