| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,093,562 | A→G | I396I (ATT→ATC) | gndA ← | NADP‑dependent phosphogluconate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,093,562 | 0 | A | G | 90.0% | 109.0 / 6.2 | 40 | I396I (ATT→ATC) | gndA | NADP‑dependent phosphogluconate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (2/2); new base G (25/11); total (27/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01 | |||||||||||
AGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTT > NZ_CP009273/2093431‑2093696 | aGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCt > 1:286465/1‑139 (MQ=255)aGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCt > 1:480406/1‑139 (MQ=255) cGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGt > 2:199234/1‑139 (MQ=255) tAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAg > 1:744694/1‑139 (MQ=255) tAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAg > 2:440857/1‑139 (MQ=255) gCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCg < 1:440857/139‑1 (MQ=255) gTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTg < 2:610494/109‑1 (MQ=255) gTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTg > 1:610494/1‑109 (MQ=255) aaTAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCtgtg > 1:457423/1‑138 (MQ=255) gCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGAtttt < 1:46955/139‑1 (MQ=255) cAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTc > 2:106144/1‑139 (MQ=255) cAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTc > 1:533221/1‑139 (MQ=255) aaCCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCg > 1:812203/1‑139 (MQ=255) aaCCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCg > 2:330279/1‑139 (MQ=255) ccGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAg > 2:277173/1‑109 (MQ=255) ccGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAg < 1:277173/109‑1 (MQ=255) ccGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCgg > 1:488143/1‑138 (MQ=255) cGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAg > 1:10631/1‑138 (MQ=255) gTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCaa < 2:111618/98‑1 (MQ=255) gTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCaa > 1:111618/1‑98 (MQ=255) ggAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGAttt < 1:625839/139‑1 (MQ=255) cGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTg < 2:480406/139‑1 (MQ=255) cTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATa > 2:532446/1‑108 (MQ=255) cTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATa < 1:532446/108‑1 (MQ=255) cTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTgcgc < 2:533221/138‑1 (MQ=255) tGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTCCTGCAGGAACTGCGCAc > 1:579399/1‑139 (MQ=255) tACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACgg < 2:286465/139‑1 (MQ=255) cATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatgat > 2:716370/1‑139 (MQ=255) cATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatgat > 2:611386/1‑139 (MQ=255) aaGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGatgatg > 2:560556/1‑137 (MQ=255) gCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGaa > 1:672854/1‑119 (MQ=255) gCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGaa < 2:672854/119‑1 (MQ=255) gCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcagc > 1:704322/1‑139 (MQ=255) gCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcagc > 2:862472/1‑139 (MQ=255) gACATCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCa > 1:600961/1‑139 (MQ=255) tCACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGa < 2:600961/139‑1 (MQ=255) cACGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGa > 2:62843/1‑138 (MQ=255) cGCAGCGCCTGCTGGTAGTCATCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGaaaa > 2:567283/1‑139 (MQ=255) aTCGGCGATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAg > 1:755863/1‑139 (MQ=255) ggCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGtt < 1:611386/138‑1 (MQ=255) | AGGTTCGCAGGCAGAACAGCAGCACGGTAGCTGTCGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTT > NZ_CP009273/2093431‑2093696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |