Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	232,843	T→C	I97T (ATC→ACC)	dnaQ →	DNA polymerase III subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	232,843	0	T	C	100.0%	102.7 / NA	28	I97T (ATC→ACC)	dnaQ	DNA polymerase III subunit epsilon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/14);  total (14/14)

ATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCG  >  NZ_CP009273/232717‑232971                                                                                                                               |                                                                                                                                 aTCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGc                                                                                                                      >  2:244134/1‑139 (MQ=255)            gATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCg                                                                                                           >  1:254709/1‑139 (MQ=255)                  gAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTa                                                                                                     <  1:628336/139‑1 (MQ=255)                        tttGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGatat                                                                                                             <  1:216126/125‑1 (MQ=255)                        tttGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGatat                                                                                                             >  2:216126/1‑125 (MQ=255)                        tttGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGAc                                                                                                >  1:142648/1‑138 (MQ=255)                                    ggTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGt                                                                                   >  2:406955/1‑139 (MQ=255)                                         tGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCt                                                                               >  2:834124/1‑138 (MQ=255)                                          gCCTATGAATTTTTGCTCTATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCtt                                                                              <  2:215022/138‑1 (MQ=255)                                           ccGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCtt                                                                              >  2:123850/1‑137 (MQ=255)                                            cGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAg                                                                           <  2:604162/139‑1 (MQ=255)                                              aTGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCa                                                                                                                        <  2:484209/92‑1 (MQ=255)                                              aTGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCa                                                                                                                        >  1:484209/1‑92 (MQ=255)                                              aTGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcg                                                                         <  2:37914/139‑1 (MQ=255)                                               tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                                                                        >  1:380516/1‑139 (MQ=255)                                                gAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                                                                        >  2:816793/1‑138 (MQ=255)                                                         cTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCg                                                               <  1:165732/138‑1 (MQ=255)                                                                aGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCaa                                                       <  1:816793/139‑1 (MQ=255)                                                                   ccACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATAc                                                    <  2:380516/139‑1 (MQ=255)                                                                           gCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGt                                             >  2:859287/1‑138 (MQ=255)                                                                               aaGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGt                                        <  1:406955/139‑1 (MQ=255)                                                                                        aTGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAg                               <  1:524828/139‑1 (MQ=255)                                                                                        aTGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAg                               <  1:244134/139‑1 (MQ=255)                                                                                                       atatTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGt                                        <  2:436779/115‑1 (MQ=255)                                                                                                       atatTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGt                                        >  1:436779/1‑115 (MQ=255)                                                                                                       atatTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAg                >  2:796609/1‑139 (MQ=255)                                                                                                       atatTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAg                >  1:26899/1‑139 (MQ=255)                                                                                                                     gAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCg  <  2:754609/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                 ATCGGCTGGTGGATCCGGAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCG  >  NZ_CP009273/232717‑232971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑