| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,196,972 | A→C | H1072P (CAC→CCC) | BW25113_RS11045 → | DUF4132 domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,196,972 | 0 | A | C | 96.4% | 88.3 / ‑3.1 | 28 | H1072P (CAC→CCC) | BW25113_RS11045 | DUF4132 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (13/14); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
GGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCACCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTCGCGCCGCACGGTGCTGCTCACACCTGACGAGTCAACCAGTAA > NZ_CP009273/2196845‑2197101 | ggggTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGaaa > 1:88894/1‑139 (MQ=255) gCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGaaa > 1:113685/1‑139 (MQ=255) tttACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGca < 2:795513/139‑1 (MQ=255) tttACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGATCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGAACCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGca > 2:498932/1‑139 (MQ=255) tACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGcac < 2:528139/138‑1 (MQ=255) gCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTg < 2:302947/139‑1 (MQ=255) tCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCTGAt > 2:158855/1‑139 (MQ=255) gCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGaaa > 2:743598/1‑139 (MQ=255) cTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGc > 2:726772/1‑139 (MQ=255) gCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGc > 2:386738/1‑137 (MQ=255) cAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGca > 1:582054/1‑97 (MQ=255) cAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGca < 2:582054/97‑1 (MQ=255) cAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAg > 2:360825/1‑139 (MQ=255) tGCGCAAGATGAGCCAATTGGCCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATg < 2:85028/79‑1 (MQ=255) tGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATg > 1:85028/1‑79 (MQ=255) cgcAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTc > 2:151127/1‑139 (MQ=255) cgcAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTc > 2:626525/1‑139 (MQ=255) gcAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTcg < 1:626525/139‑1 (MQ=255) aGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTCGCGCCGCa < 2:46025/138‑1 (MQ=255) aGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTCGCGCCGCa < 2:549075/138‑1 (MQ=255) aGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTCGCGCCGCa < 1:657089/138‑1 (MQ=255) aGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTCGCGCCGCAc > 2:222463/1‑139 (MQ=255) aaTTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCACCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTCGCGCCGCACGGtg < 2:472492/139‑1 (MQ=255) aCCTGCCTGCGGACGCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTCGCGCCGCACGGTgctgct < 1:769890/139‑1 (MQ=255) gCTCTGATTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTCGCGCCGCACGGTGCTGCTCACACCTGACGAGt < 1:386738/139‑1 (MQ=255) aTTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTCGCGCCGCACGGTGCTGCTCACACCTGACGAGTCAAc < 1:743598/137‑1 (MQ=255) aTTGGCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTCGCGCCGCACGGTGCTGCTCACACCTGACGAGTCAAc < 1:360825/137‑1 (MQ=255) gCATTGCCCCCCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTCGCGCCGCACGGTGCTGCTCACACCTGACGAGTCAACCAGTaa < 2:72971/139‑1 (MQ=255) | GGGGTTTATCCGGCAAATGAACCGCGTTGTTTACTCAAAGCCTTTCGTGTGGCCGCAGAGGGGGAGTTCTGCAATGCGCAAGATGAGCCAATTGACCTGCCTGCGGACGCTCTGATTGGCATTGCCCACCCGTTAGAAATGACAGCAGAAATGCGCAGTGAATTTGCACAGCTTTTTGCCGATTACGAAATTATGCCGCCTTTTCGCCAGTTGTCGCGCCGCACGGTGCTGCTCACACCTGACGAGTCAACCAGTAA > NZ_CP009273/2196845‑2197101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |