Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,268,854	A→G	S35G (AGT→GGT)	yejE →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,268,854	0	A	G	100.0%	97.4 / NA	30	S35G (AGT→GGT)	yejE	ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/16);  total (14/16)

TGGTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGAGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGA  >  NZ_CP009273/2268718‑2268981                                                                                                                                         |                                                                                                                                tGGTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGt                                                                                                                               >  1:78846/1‑139 (MQ=255)   gTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt                                                                                                                             >  1:177425/1‑139 (MQ=255)   gTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt                                                                                                                             >  2:280912/1‑139 (MQ=255)   gTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt                                                                                                                             <  2:218018/139‑1 (MQ=255)   gTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAAACAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt                                                                                                                             >  1:376972/1‑139 (MQ=255)      gATCCGCGTATTGATTTTGTGGGACGTTACTGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGACATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGCTTGGTTTGGGTTtgtg                                                                                                                          >  2:65834/1‑139 (MQ=255)        tCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGtt                                                                                                                        >  2:528108/1‑139 (MQ=255)        tCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGtt                                                                                                                        >  1:742693/1‑139 (MQ=255)                       tGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGcc                                                                                                          <  1:280912/138‑1 (MQ=255)                       tGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGcc                                                                                                          <  2:78846/138‑1 (MQ=255)                       tGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGcc                                                                                                          <  2:73020/138‑1 (MQ=255)                            gACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGAt                                                                                                    <  1:692492/139‑1 (MQ=255)                              cGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATaa                                                                                                  <  1:4645/139‑1 (MQ=255)                                         cgACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt                                                                                                                             <  2:398343/101‑1 (MQ=255)                                         cgACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt                                                                                                                             >  1:398343/1‑101 (MQ=255)                                                      tCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGcc                                                                                                          >  1:775552/1‑107 (MQ=255)                                                      tCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGcc                                                                                                          <  2:775552/107‑1 (MQ=255)                                                      tCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACgg                                                                          >  2:425944/1‑139 (MQ=255)                                                            aGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGtt                                                                     <  1:398759/138‑1 (MQ=255)                                                                        cgcgTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt                                                                                                                             <  2:31025/70‑1 (MQ=255)                                                                        cgcgTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGttt                                                                                                                             >  1:31025/1‑70 (MQ=255)                                                                          cgTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTAt                                                      <  2:79106/139‑1 (MQ=255)                                                                           gTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTAtt                                                     >  1:46510/1‑139 (MQ=255)                                                                                cGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGaaa                                                 <  2:376972/138‑1 (MQ=255)                                                                                                       gTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTggc                         <  2:676568/139‑1 (MQ=255)                                                                                                         cGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTggcgg                       >  2:306790/1‑139 (MQ=255)                                                                                                                      ccTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGt          >  2:291735/1‑139 (MQ=255)                                                                                                                           tCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGc     <  1:406389/139‑1 (MQ=255)                                                                                                                               gtttggtttgGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGa  <  2:742693/138‑1 (MQ=255)                                                                                                                               gtttggtttgGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGa  <  2:843199/138‑1 (MQ=255)                                                                                                                                         |                                                                                                                                TGGTTGATCCGCGTATTGATTTTGAGGGACGTTAATGTCGCGACTCAGCCCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGAGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAAACTACAGCGAAAGCGATTTTGGCGGCCCGCTGGCAAGTCAGGCTGA  >  NZ_CP009273/2268718‑2268981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑