Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,303,411	T→C	G158G (GGA→GGG)	ada ←	bifunctional DNA‑binding transcriptional regulator/O6‑methylguanine‑DNA methyltransferase Ada

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,303,411	0	T	C	100.0%	154.1 / NA	44	G158G (GGA→GGG)	ada	bifunctional DNA‑binding transcriptional regulator/O6‑methylguanine‑DNA methyltransferase Ada
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/22);  total (22/22)

ACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAATCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAA  >  NZ_CP009273/2303277‑2303545                                                                                                                                       |                                                                                                                                       accaGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCgg                                                                                                                                    >  2:375214/1‑139 (MQ=255)   caGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCg                                                                                                                                  >  2:439364/1‑139 (MQ=255)     ggCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGt                                                                                                                                 >  1:674627/1‑138 (MQ=255)          cGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGaa                                                                                                                           >  1:644873/1‑139 (MQ=255)          cGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGaa                                                                                                                           <  1:470025/139‑1 (MQ=255)              ccAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTACGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGa                                                                                                                       <  2:530328/139‑1 (MQ=255)                aGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGAcg                                                                                                                     >  1:787648/1‑139 (MQ=255)                       aaTCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCAc                                                                                                              >  2:814926/1‑139 (MQ=255)                         tCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGc                                                                                                            <  1:375214/139‑1 (MQ=255)                            gccagcGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGctct                                                                                                         >  1:630230/1‑139 (MQ=255)                                 cgcgTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCt                                                                                                    <  1:865091/139‑1 (MQ=255)                                     tAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCg                                                                                                >  1:112353/1‑139 (MQ=255)                                           aCCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGc                                                                                           >  2:704806/1‑138 (MQ=255)                                            ccGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGa                                                                                                                       <  2:373376/109‑1 (MQ=255)                                            ccGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGa                                                                                                                       >  1:373376/1‑109 (MQ=255)                                             cGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGAt                                                                                        >  1:585519/1‑139 (MQ=255)                                                    ttttCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCa                                                                                 <  1:814926/139‑1 (MQ=255)                                                       tCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAAc                                                                              >  2:630498/1‑139 (MQ=255)                                                            aCCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGa                                                                         <  2:157490/139‑1 (MQ=255)                                                             ccGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAg                                                                        <  2:683538/139‑1 (MQ=255)                                                             ccGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAg                                                                        >  1:142708/1‑139 (MQ=255)                                                             ccGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAg                                                                        <  2:491108/139‑1 (MQ=255)                                                             ccGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAg                                                                        <  1:465498/139‑1 (MQ=255)                                                             ccGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAg                                                                        <  1:712161/139‑1 (MQ=255)                                                                             tAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTg                                                        >  2:111767/1‑139 (MQ=255)                                                                               gCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGc                                                       <  1:704806/138‑1 (MQ=255)                                                                                          cAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGc                                           <  1:439364/139‑1 (MQ=255)                                                                                              gTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCa                                       <  2:674627/139‑1 (MQ=255)                                                                                                ttCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGAcg                                                                                                                     >  2:516711/1‑59 (MQ=255)                                                                                                ttCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGAcg                                                                                                                     <  1:516711/59‑1 (MQ=255)                                                                                                       gCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAg                              <  1:104781/139‑1 (MQ=255)                                                                                                       gCTTTGCGATAGTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAg                              >  1:238257/1‑139 (MQ=255)                                                                                                                  gTAACTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTaaa                    >  2:524722/1‑138 (MQ=255)                                                                                                                     aCTGCTGCTGTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAaacaac                <  1:566948/139‑1 (MQ=255)                                                                                                                        gctgctgTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGa             >  1:333119/1‑139 (MQ=255)                                                                                                                         ctgctgTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGAt            >  1:51757/1‑139 (MQ=255)                                                                                                                             tgTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGc                                                       >  2:253441/1‑92 (MQ=255)                                                                                                                             tgTCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGc                                                       <  1:253441/92‑1 (MQ=255)                                                                                                                               tCGGGGAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAg        <  2:644873/137‑1 (MQ=255)                                                                                                                                 ggggAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGAtt                                                                                       <  2:297356/56‑1 (MQ=255)                                                                                                                                 ggggAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGAtt                                                                                       >  1:297356/1‑56 (MQ=255)                                                                                                                                 ggggAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATg     >  2:126767/1‑138 (MQ=255)                                                                                                                                  gggAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGaa   <  1:126767/139‑1 (MQ=255)                                                                                                                                   ggAACCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGaaa  <  2:238257/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       ACCAGGCAACGACCCAGCTCACAATCAGCCAGCGCGTAACGCACCGCCAGATTTTCGCCACCGTGACGGAATTGTTTAGCCGTCATGCCCAGCGTTTCGTCAGCTTTGCGATAGTAACTGCTGCTGTCGGGGAATCCGGCGTTAAGAATAGACGTCGTCACGCTCTCCCCTTTCGCCAGCGATTCGCGCAAACGGCGAGCGCGCCAGGCCTGTTGCCAGGCTTTAGGCGTCATTCCGGTAGTCGCTTTAAACAACCGATGTAGATGAAA  >  NZ_CP009273/2303277‑2303545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑