Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,357,677	A→G	G26G (GGT→GGC)	yfaZ ←	YfaZ family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,357,677	0	A	G	100.0%	126.9 / NA	38	G26G (GGT→GGC)	yfaZ	YfaZ family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/19);  total (19/19)

CAGATTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCC  >  NZ_CP009273/2357545‑2357812                                                                                                                                     |                                                                                                                                        cAGATTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCt                                                                                                                                    >  1:253026/1‑138 (MQ=255)   gATTCACCCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGCCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGAt                                                                                                                                 <  2:95390/139‑1 (MQ=255)   gATTCAACCCCAGCCCCACGCCAGCGACATCCCCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGAt                                                                                                                                 <  1:411442/139‑1 (MQ=255)   gATTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGAt                                                                                                                                 <  1:341481/139‑1 (MQ=255)                       ccAGCGACAGCCCGGTCGTCGCCGTTAGGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGGCCCAAAACCGACACCAATATTGGTGTATTCTTTGCCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATg                                                                                                             <  1:582315/139‑1 (MQ=255)                                    cgtcgtcgtcgtTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAg                                                                                                          >  1:43513/1‑129 (MQ=255)                                    cgtcgtcgtcgtTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAg                                                                                                          <  2:43513/129‑1 (MQ=255)                                                      tCCAGTTACCGCTTAACGCCAGGCCCCTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGAATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGCCCGATGCAGAAACCAACAGCATTCCGGCCAGACCTgt                                                                              >  2:678120/1‑138 (MQ=255)                                                       ccAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGc                                                                              >  2:687944/1‑138 (MQ=255)                                                                gCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCa                                                                                                           <  1:514190/100‑1 (MQ=255)                                                                gCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCa                                                                                                           >  2:514190/1‑100 (MQ=255)                                                                 cTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAttt                                                                   >  2:434209/1‑139 (MQ=255)                                                                     aCGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAttttttt                                                               >  1:82157/1‑139 (MQ=255)                                                                         cAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCAtt                                                           <  1:434209/139‑1 (MQ=255)                                                                         cAGGCCCGTCGTTTCAGGCCAAAATCCGACGCCAATATTGGTGTATTCTTTGCCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCAtt                                                           <  2:253026/139‑1 (MQ=255)                                                                                            ccAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGAt                                                                                                                                 <  1:40345/50‑1 (MQ=255)                                                                                            ccAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGAt                                                                                                                                 >  2:40345/1‑50 (MQ=255)                                                                                                    gACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACTAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATc                                 >  2:375422/1‑138 (MQ=255)                                                                                                    gACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATc                                 >  2:474114/1‑138 (MQ=255)                                                                                                      ctcaaaTATTGGTTTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATGCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCaaa                              <  1:678120/135‑1 (MQ=255)                                                                                                                   gtATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTg                 >  2:221283/1‑139 (MQ=255)                                                                                                                   gtATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTg                 >  1:680322/1‑139 (MQ=255)                                                                                                                    tATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTg                 <  1:104375/138‑1 (MQ=255)                                                                                                                    tATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTg                 <  2:823825/138‑1 (MQ=255)                                                                                                                    tATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTg                 <  2:468472/138‑1 (MQ=255)                                                                                                                      ttCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTa                                                          >  1:604968/1‑95 (MQ=255)                                                                                                                      ttCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTa                                                          <  2:604968/95‑1 (MQ=255)                                                                                                                      ttCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTg                 <  1:162907/136‑1 (MQ=255)                                                                                                                      ttCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTg                 >  2:162907/1‑136 (MQ=255)                                                                                                                           tACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTg         >  2:760916/1‑139 (MQ=255)                                                                                                                           tACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTg         >  2:398835/1‑139 (MQ=255)                                                                                                                             cccGCCTGGCCGCTGATGCTCATTGCATTGACCTATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTGTCATTATCCCCTTGCCCACAAGCAATTAAACAAAATTCCCAAAGCTGCGGGATTGTa       <  1:375422/139‑1 (MQ=255)                                                                                                                             cccGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTa       <  2:680322/139‑1 (MQ=255)                                                                                                                                gCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTg    >  1:652570/1‑139 (MQ=255)                                                                                                                                gCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTg    >  2:792954/1‑139 (MQ=255)                                                                                                                                 ccTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGc   <  1:751063/139‑1 (MQ=255)                                                                                                                                 ccTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGc   >  2:124356/1‑139 (MQ=255)                                                                                                                                  cTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGcc  <  2:82157/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        CAGATTCAACCCCAGCCCCACGCCAGCGACATCACCGTCGTCGTCGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCTTTCCCACAAGCAATTAATCAAAATTCCCAAAGCTGCGGGATTGTACTGCC  >  NZ_CP009273/2357545‑2357812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑