Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	243,919	A→T	R241W (AGG→TGG)	yafL →	C40 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	243,919	0	A	T	100.0%	185.3 / NA	45	R241W (AGG→TGG)	yafL	C40 family peptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (25/20);  total (25/20)

CGAGATAGCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCG  >  NZ_CP009273/243789‑244052                                                                                                                                   |                                                                                                                                      cgAGATAGCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGAtttt                                                                                                                                >  1:117936/1‑138 (MQ=255) cgAGATAGCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGAtttt                                                                                                                                >  2:373753/1‑138 (MQ=255)         ccGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGaa                                                                                                                         >  2:96526/1‑137 (MQ=255)         ccGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAaga                                                                                                                       <  2:569258/139‑1 (MQ=255)              cATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGa                                                                                                                  >  2:698180/1‑139 (MQ=255)               atatGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGa                                                                                                                  <  1:194741/138‑1 (MQ=255)                   gggCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTg                                                                                                             >  1:384156/1‑139 (MQ=255)                      cGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAg                                                                                                          >  1:147042/1‑139 (MQ=255)                        tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAgg                                                                                                         <  1:8479/138‑1 (MQ=255)                        tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAgg                                                                                                         >  2:8479/1‑138 (MQ=255)                        tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                        >  1:332122/1‑139 (MQ=255)                        tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                        >  1:787929/1‑139 (MQ=255)                        tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                        >  1:775423/1‑139 (MQ=255)                        tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGa                                                                                                        >  2:94207/1‑139 (MQ=255)                          tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGAc                                                                                                       <  2:256337/138‑1 (MQ=255)                                ggCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATaa                                                                                                 <  2:384156/138‑1 (MQ=255)                                    aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGt                                                                                            >  2:77571/1‑139 (MQ=255)                                    aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGt                                                                                            >  1:453875/1‑139 (MQ=255)                                    aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGt                                                                                            >  2:213004/1‑139 (MQ=255)                                               aTCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCa                                                                                  <  1:373753/138‑1 (MQ=255)                                               aTCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCa                                                                                  <  1:94207/138‑1 (MQ=255)                                                   aGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCgg                                                                             <  2:426323/139‑1 (MQ=255)                                                      cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                          >  2:665887/1‑139 (MQ=255)                                                       gCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                          <  2:453875/138‑1 (MQ=255)                                                       gCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAg                                                                          <  1:181061/138‑1 (MQ=255)                                                       gCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGt                                                                         >  2:619929/1‑139 (MQ=255)                                                        ccACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGtt                                                                        <  1:77571/139‑1 (MQ=255)                                                             tACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACg                                                                   <  2:332122/139‑1 (MQ=255)                                                                  gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAgaga                                                                                                                     >  2:19541/1‑84 (MQ=255)                                                                  gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAgaga                                                                                                                     <  1:19541/84‑1 (MQ=255)                                                                  gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGt                                                              >  2:215426/1‑139 (MQ=255)                                                                       aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCt                                                          >  1:178058/1‑138 (MQ=255)                                                                       aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCt                                                          >  1:385361/1‑138 (MQ=255)                                                                                   aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGa                                                                                                                              >  1:7688/1‑58 (MQ=255)                                                                                   aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGa                                                                                                                              <  2:7688/58‑1 (MQ=255)                                                                                    gCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                            <  1:345389/139‑1 (MQ=255)                                                                                      cGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcgc                                          <  2:844354/139‑1 (MQ=255)                                                                                            gCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTa                                    <  2:117936/139‑1 (MQ=255)                                                                                              cGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                  <  1:213004/139‑1 (MQ=255)                                                                                              cGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                  <  2:787929/139‑1 (MQ=255)                                                                                                 aCCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATg                               >  2:593710/1‑139 (MQ=255)                                                                                                             ggACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCgg                                                                             <  1:83954/81‑1 (MQ=255)                                                                                                             ggACCATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCgg                                                                             >  2:83954/1‑81 (MQ=255)                                                                                                                ccATTTTTTGGGCGCGCGCTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgcg                 >  1:501661/1‑138 (MQ=255)                                                                                                                              cgcgcTGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCg  >  1:221538/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                      CGAGATAGCCGATCATATGGGCGTGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCG  >  NZ_CP009273/243789‑244052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑