Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,406,008	A→G	D22D (GAT→GAC)	yfbU ←	YfbU family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,406,008	0	A	G	100.0%	197.7 / NA	56	D22D (GAT→GAC)	yfbU	YfbU family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/26);  total (30/26)

TAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTA  >  NZ_CP009273/2405878‑2406135                                                                                                                                   |                                                                                                                                tAATGTCGTTGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCa                                                                                                                          >  1:761395/1‑138 (MQ=255)    tGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGt                                                                                                                      >  2:665757/1‑139 (MQ=255)      tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                    <  1:109181/139‑1 (MQ=255)      tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                    <  2:318662/139‑1 (MQ=255)      tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                    <  1:347414/139‑1 (MQ=255)      tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                    <  2:346274/139‑1 (MQ=255)      tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                    >  2:53021/1‑139 (MQ=255)      tCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                    <  2:539020/139‑1 (MQ=255)         atgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtcat                                                                                                                   >  2:746743/1‑137 (MQ=255)         atgatAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtcat                                                                                                                   <  1:746743/137‑1 (MQ=255)                tGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTa                                                                                                          >  1:110920/1‑139 (MQ=255)                tGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTa                                                                                                          >  1:95368/1‑139 (MQ=255)                tGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTa                                                                                                          >  1:580339/1‑139 (MQ=255)                  cGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                    >  2:733133/1‑127 (MQ=255)                  cGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                    <  1:733133/127‑1 (MQ=255)                                  tCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAAt                                                                                           >  1:588157/1‑136 (MQ=255)                                   cAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAg                                                                                        <  2:231665/138‑1 (MQ=255)                                         gCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAATACGTTgc                                                                                 >  1:780945/1‑139 (MQ=255)                                         gCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGtt                                                                                   <  1:166150/137‑1 (MQ=255)                                                              aCGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgtt                                                            <  1:53021/139‑1 (MQ=255)                                                               cGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgttg                                                           <  2:588157/139‑1 (MQ=255)                                                                gCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgtt                                                            <  2:457267/137‑1 (MQ=255)                                                                gCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCAttgtt                                                            >  1:457267/1‑137 (MQ=255)                                                                     tGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCt                                                     >  2:427208/1‑139 (MQ=255)                                                                            cGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGa                                                                                                      >  1:676344/1‑83 (MQ=255)                                                                            cGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGa                                                                                                      <  2:676344/83‑1 (MQ=255)                                                                            cGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTAt                                                 <  1:304725/136‑1 (MQ=255)                                                                            cGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTAt                                                 >  2:304725/1‑136 (MQ=255)                                                                            cGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCa                                               <  2:631137/138‑1 (MQ=255)                                                                            cGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCAc                                              >  1:631137/1‑139 (MQ=255)                                                                              tAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTc                                                                   >  1:78142/1‑116 (MQ=255)                                                                              tAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTc                                                                   <  2:78142/116‑1 (MQ=255)                                                                              tAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCActc                                            >  1:208744/1‑139 (MQ=255)                                                                              tAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCActc                                            >  2:840922/1‑139 (MQ=255)                                                                              tAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCActc                                            >  2:657915/1‑139 (MQ=255)                                                                              tAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCActc                                            >  1:68075/1‑139 (MQ=255)                                                                              tAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCActc                                            >  2:4426/1‑139 (MQ=255)                                                                              tAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCActc                                            >  1:617019/1‑139 (MQ=255)                                                                              tAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCActc                                            >  1:120712/1‑139 (MQ=255)                                                                                    cGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                    <  2:331644/61‑1 (MQ=255)                                                                                    cGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGtca                                                                                                                    >  1:331644/1‑61 (MQ=255)                                                                                      cTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACgg                                    >  1:840662/1‑139 (MQ=255)                                                                                      cTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACgg                                    >  1:341192/1‑139 (MQ=255)                                                                                         aataatTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGt                                   <  1:321266/137‑1 (MQ=255)                                                                                                 tttGCAGGCGACGGTAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGaaaaaa                            >  2:166937/1‑136 (MQ=255)                                                                                                               tAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGc           <  2:580339/139‑1 (MQ=255)                                                                                                               tAACGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGc           <  1:166937/139‑1 (MQ=255)                                                                                                                 aCGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGcc          <  1:657915/138‑1 (MQ=255)                                                                                                                  cGTTCAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCctt        <  1:77846/139‑1 (MQ=255)                                                                                                                      cAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTg                                                    <  1:117654/91‑1 (MQ=255)                                                                                                                      cAGCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTg                                                    >  2:117654/1‑91 (MQ=255)                                                                                                                        gCATTTGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTta  <  2:341192/139‑1 (MQ=255)                                                                                                                            ttGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGt                                                                         <  2:560097/64‑1 (MQ=255)                                                                                                                            ttGCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGt                                                                         >  1:560097/1‑64 (MQ=255)                                                                                                                              gCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCa                                               <  2:688328/88‑1 (MQ=255)                                                                                                                              gCCGGGTCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCa                                               >  1:688328/1‑88 (MQ=255)                                                                                                                                   |                                                                                                                                TAATGTCGATGATAGTGCGGCAGGTTTCTTCTTTCAGCTCGCCAAACTCGCGATCCAATTCACGCATCTGTAATCCGTAACCACGCTCAATAATTGTTTGCAGGCGACGGTAACGTTCAGCATTTGCCGGATCGAGCATAGTCATCATCTTGTACTGATTAGACAAAATAAGACGTTGCGCGTTGGTCATTTCCATTGTTGACTCCTGTATCACTCTACTACGGTGAAAAAAAAGAAGGCTGAGTATGCCTTCTTTTA  >  NZ_CP009273/2405878‑2406135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑