| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,437,066 | T→C | D608D (GAT→GAC) | mnmC → | bifunctional tRNA (5‑methylaminomethyl‑2‑thiouridine)(34)‑methyltransferase MnmD/FAD‑dependent 5‑carboxymethylaminomethyl‑2‑thiouridine(34) oxidoreductase MnmC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,437,066 | 0 | T | C | 92.1% | 110.4 / 0.0 | 38 | D608D (GAT→GAC) | mnmC | bifunctional tRNA (5‑methylaminomethyl‑2‑thiouridine)(34)‑methyltransferase MnmD/FAD‑dependent 5‑carboxymethylaminomethyl‑2‑thiouridine(34) oxidoreductase MnmC |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (20/15); total (21/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-01 | |||||||||||
CGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAA > NZ_CP009273/2436929‑2437197 | cgcTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGAt > 1:13542/1‑138 (MQ=255)cgcTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGAt < 2:13542/138‑1 (MQ=255) tgCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCt > 2:746450/1‑139 (MQ=255) cccGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTTTGTTTGAGGGTTTAgg > 2:8432/1‑139 (MQ=255) cccGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAgg > 1:448005/1‑139 (MQ=255) gATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGGtctc > 1:863349/1‑139 (MQ=255) tcatcTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTcgc < 1:8432/139‑1 (MQ=255) aTGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGc > 1:326240/1‑113 (MQ=255) aTGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGc < 2:326240/113‑1 (MQ=255) ggTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCt > 1:273572/1‑139 (MQ=255) ggCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGcc < 2:748361/138‑1 (MQ=255) gCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGcccc > 2:833600/1‑139 (MQ=255) aTGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCt < 1:110189/139‑1 (MQ=255) aTGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCt < 1:833600/139‑1 (MQ=255) tGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCtg < 2:863349/139‑1 (MQ=255) acacTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTggcgg < 2:273572/139‑1 (MQ=255) acTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGgcggcg < 1:468739/139‑1 (MQ=255) tGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTtct < 2:413665/89‑1 (MQ=255) tGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTtct > 1:413665/1‑89 (MQ=255) tGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGc > 1:1713/1‑111 (MQ=255) tGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGc < 2:1713/111‑1 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGAt > 1:587490/1‑55 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGAt < 2:587490/55‑1 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCt > 1:216281/1‑105 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCt < 2:216281/105‑1 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGcgac > 1:112227/1‑139 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGcgac > 2:652679/1‑139 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGcgac > 2:263957/1‑139 (MQ=255) tGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGGTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGcgac > 1:766601/1‑139 (MQ=255) tGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTcgc < 1:849825/77‑1 (MQ=255) tGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTcgc > 2:849825/1‑77 (MQ=255) gCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCgatg > 1:731499/1‑139 (MQ=255) aGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCgatgga < 1:393704/139‑1 (MQ=255) aGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGtt < 2:479661/69‑1 (MQ=255) aGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGtt > 1:479661/1‑69 (MQ=255) aGGCGGTAAGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCGATGGATGCCAGTAc > 1:35577/1‑139 (MQ=255) aaGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCgatgga < 2:653473/123‑1 (MQ=255) aaGCGCGCCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCgatgga > 1:653473/1‑123 (MQ=255) cgcCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGt > 2:230051/1‑138 (MQ=255) cgcCGGTTTTTGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGt > 1:473281/1‑138 (MQ=255) tGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGaa < 2:35577/138‑1 (MQ=255) tGACGACCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGaa < 2:473281/138‑1 (MQ=255) | CGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACTCGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATCTCTTTATGTTTGCGGCTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCGATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAA > NZ_CP009273/2436929‑2437197 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |