Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,503,481	A→G	T125A (ACC→GCC)	yfeO →	ion channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,503,481	0	A	G	100.0%	97.2 / NA	28	T125A (ACC→GCC)	yfeO	ion channel protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/14);  total (14/14)

ATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGGCACTCCTGTTGCGGCGGCGTTGA  >  NZ_CP009273/2503347‑2503613                                                                                                                                       |                                                                                                                                     aTGCCGGACCAGCCCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCg                                                                                                                                   <  1:670359/138‑1 (MQ=255)                 cGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCg                                                                                                                                   <  2:245642/122‑1 (MQ=255)                              cTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGgcg                                                                                                     >  1:238699/1‑139 (MQ=255)                              cTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGgcg                                                                                                     >  1:794205/1‑139 (MQ=255)                                 aTCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGgcgc                                                                                                    <  2:243646/137‑1 (MQ=255)                                         aCCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTa                                                                                          >  1:641267/1‑139 (MQ=255)                                                ccGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTAc                                                                                         >  1:151245/1‑133 (MQ=255)                                                ccGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTAc                                                                                         <  2:151245/133‑1 (MQ=255)                                                ccGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACcgcgcg                                                                                   >  1:596719/1‑139 (MQ=255)                                                        tGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGgcgc                                                                                                    <  1:473170/114‑1 (MQ=255)                                                        tGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGgcgc                                                                                                    >  2:473170/1‑114 (MQ=255)                                                        tGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCg                                                                            >  2:771366/1‑138 (MQ=255)                                                               ccTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAg                                                                     <  2:641267/138‑1 (MQ=255)                                                               ccTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGAGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGa                                                                      <  1:460169/137‑1 (MQ=255)                                                                   gACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGa                                                                 <  2:244768/138‑1 (MQ=255)                                                                    aCTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGgcgc                                                                                                    >  2:117471/1‑102 (MQ=255)                                                                    aCTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGgcgc                                                                                                    <  1:117471/102‑1 (MQ=255)                                                                    aCTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGAc                                                                >  2:530088/1‑138 (MQ=255)                                                                        aTCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTAttt                                                           >  1:474569/1‑139 (MQ=255)                                                                         tCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCaa                                                                               >  1:611706/1‑118 (MQ=255)                                                                         tCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCaa                                                                               <  2:611706/118‑1 (MQ=255)                                                                         tCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTAttt                                                           >  2:603811/1‑138 (MQ=255)                                                                              gCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGcc                                                      <  1:530088/138‑1 (MQ=255)                                                                              gCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCct                                                     <  2:596719/139‑1 (MQ=255)                                                                                     ttCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCgg                                              <  1:229752/139‑1 (MQ=255)                                                                                         cGGTCTTGCTGGCGGCGTCAGCCTGGGACCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGAGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAAACGAATGGAGTGGACTATTTTAGCCTCTGCCGGAAc                                           >  2:579341/1‑138 (MQ=255)                                                                                                          tCAGCCTGGGGCCGGAACATCCGATCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTgg                         <  1:771366/139‑1 (MQ=255)                                                                                                                                 aTCATGGCCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGGCACTCCTGTTGCGGCGGCGTTGa  >  2:849981/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                     ATGCCGGACCAGACCCCGCCTGTGAACCGCTGATCGGCGCACCGGTTCCGCCCTCTGCGCTACCTGGACTTATCGTAGCATTAATTCTCGGTCTTGCTGGCGGCGTCAGCCTGGGGCCGGAACATCCGATCATGACCGTCAATATCGCCCTTGCGGTTGCCATTGGCGCTCGTCTGTTACCGCGCGTCAACCGAATGGAGTGGACTATTTTAGCCTCTGCCGGAACCATCGGCGCACTGTTTGGCACTCCTGTTGCGGCGGCGTTGA  >  NZ_CP009273/2503347‑2503613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑