Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,521,647	C→T	G630D (GGC→GAC)	ligA ←	NAD‑dependent DNA ligase LigA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,521,647	0	C	T	100.0%	146.2 / NA	36	G630D (GGC→GAC)	ligA	NAD‑dependent DNA ligase LigA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/16);  total (20/16)

AGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGCCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATC  >  NZ_CP009273/2521522‑2521778                                                                                                                              |                                                                                                                                    aGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACttt                                                                                                                           <  2:345574/136‑1 (MQ=255) aGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTcgc                                                                                                                        >  2:549828/1‑139 (MQ=255)           cAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTc                                                                                                               >  2:745948/1‑138 (MQ=255)           cAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTc                                                                                                               >  2:469201/1‑138 (MQ=255)                gcagcaTTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAg                                                                                                         >  2:7165/1‑139 (MQ=255)                  agcaTTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTc                                                                                                       <  2:542962/139‑1 (MQ=255)                   gcaTTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTc                                                                                                       >  1:704854/1‑138 (MQ=255)                   gcaTTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCg                                                                                                      <  1:7165/139‑1 (MQ=255)                         ccGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCtt                                                                                                 >  2:710848/1‑138 (MQ=255)                                 tcgATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTcgcg                                                                                                                       >  2:93781/1‑108 (MQ=255)                                 tcgATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTcgcg                                                                                                                       <  1:93781/108‑1 (MQ=255)                                     tGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCAcg                                                                                    <  1:21094/139‑1 (MQ=255)                                            aaTGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACAt                                                                              <  2:704854/138‑1 (MQ=255)                                            aaTGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACAt                                                                              <  2:758240/138‑1 (MQ=255)                                                   aGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCtt                                                                       >  1:784605/1‑138 (MQ=255)                                                          gcgcCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGcc                                                               >  1:414483/1‑139 (MQ=255)                                                          gcgcCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGcc                                                               >  1:677709/1‑139 (MQ=255)                                                             ccTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGt                                                            <  1:35224/139‑1 (MQ=255)                                                               ttCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGAc                                                                                                            <  2:414239/89‑1 (MQ=255)                                                               ttCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGAc                                                                                                            >  1:414239/1‑89 (MQ=255)                                                               ttCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGt                                                            >  2:35224/1‑137 (MQ=255)                                                               ttCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGCTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTACGCTGCCCGt                                                            >  2:145431/1‑137 (MQ=255)                                                                               cTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTAcc                                          <  2:509072/139‑1 (MQ=255)                                                                                tGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACAt                                                                              <  2:317928/102‑1 (MQ=255)                                                                                tGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACAt                                                                              >  1:317928/1‑102 (MQ=255)                                                                                 gCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAg                                        >  2:268784/1‑139 (MQ=255)                                                                                         aCCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGGCCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACggg                                <  2:784605/139‑1 (MQ=255)                                                                                         aCCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACggg                                <  1:39898/139‑1 (MQ=255)                                                                                            cGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTg                             <  2:725670/139‑1 (MQ=255)                                                                                               tATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTc                           >  2:79484/1‑138 (MQ=255)                                                                                                        aTCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTcc                 >  2:517703/1‑139 (MQ=255)                                                                                                        aTCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTcc                 >  2:468034/1‑139 (MQ=255)                                                                                                                 cttcGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGAt         <  1:268784/138‑1 (MQ=255)                                                                                                                    cGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAAc      >  1:818528/1‑138 (MQ=255)                                                                                                                      acacGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGAt   <  1:145431/139‑1 (MQ=255)                                                                                                                       cacGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATc  >  2:485971/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                    AGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGCCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGTTTTACCAGCAAACGGGCTGTCAATCTCTTCCGCGTTGATAACGATC  >  NZ_CP009273/2521522‑2521778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑