Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,550,584	A→G	S25P (TCA→CCA)	eutC ←	ethanolamine ammonia‑lyase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,550,584	0	A	G	100.0%	137.5 / NA	37	S25P (TCA→CCA)	eutC	ethanolamine ammonia‑lyase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/17);  total (20/17)

CTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCG  >  NZ_CP009273/2550452‑2550718                                                                                                                                     |                                                                                                                                       cTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGgcggcg                                                                                                                                   <  1:354695/138‑1 (MQ=255)      aaTACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCgggg                                                                                                                              <  2:195541/138‑1 (MQ=255)         aCGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCg                                                                                                                          >  1:196538/1‑139 (MQ=255)         aCGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCg                                                                                                                          >  1:479253/1‑139 (MQ=255)          cGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGc                                                                                                                         <  2:479253/139‑1 (MQ=255)                cgcgATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTcc                                                                                                                   >  1:545314/1‑139 (MQ=255)                  cgATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCa                                                                                                                 <  1:396579/139‑1 (MQ=255)                       cGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGa                                                                                                             <  2:44055/138‑1 (MQ=255)                                  cacCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCt                                                                                                 <  1:31085/139‑1 (MQ=255)                                      aaTCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGt                                                                                             <  2:338497/139‑1 (MQ=255)                                                 ttGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATttctt                                                                                   >  2:53716/1‑138 (MQ=255)                                                  tGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATttcttc                                                                                  >  2:685416/1‑138 (MQ=255)                                                       cAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATc                                                                              <  2:196538/137‑1 (MQ=255)                                                           ggAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGtttt                                                                        <  2:545252/139‑1 (MQ=255)                                                                   aaTCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCAt                                                                <  2:540444/139‑1 (MQ=255)                                                                       cAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGata                                                            >  1:441770/1‑139 (MQ=255)                                                                       cAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGata                                                            >  1:749636/1‑139 (MQ=255)                                                                         gcgcgcAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGcgcg                                                                                                                                 >  1:35306/1‑68 (MQ=255)                                                                         gcgcgcAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGcgcg                                                                                                                                 <  2:35306/68‑1 (MQ=255)                                                                         gcgcACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGa                                                              <  1:263916/135‑1 (MQ=255)                                                                         gcgcACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGa                                                              >  2:263916/1‑135 (MQ=255)                                                                          cgcACAGCTTTCCGAGGTGACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGt                                                         >  2:484357/1‑139 (MQ=255)                                                                                     ccGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCcgcg                                               <  1:77760/138‑1 (MQ=255)                                                                                        aGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGtcat                                           <  2:441770/139‑1 (MQ=255)                                                                                         ggTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGtcat                                           >  2:559703/1‑138 (MQ=255)                                                                                           tCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCgggg                                                                                                                              >  1:544261/1‑53 (MQ=255)                                                                                           tCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGAGCgggg                                                                                                                              <  2:544261/53‑1 (MQ=255)                                                                                                            ttGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACg                           <  2:139485/135‑1 (MQ=255)                                                                                                            ttGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACg                           >  1:139485/1‑135 (MQ=255)                                                                                                            ttGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATc                       >  1:726925/1‑139 (MQ=255)                                                                                                                      cgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATc                                                                              >  2:515311/1‑74 (MQ=255)                                                                                                                      cgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATc                                                                              <  1:515311/74‑1 (MQ=255)                                                                                                                      cgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGt             >  2:498367/1‑139 (MQ=255)                                                                                                                      cgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGt             >  2:404688/1‑139 (MQ=255)                                                                                                                      cgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGt             >  1:528890/1‑139 (MQ=255)                                                                                                                      cgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGt             >  1:90561/1‑139 (MQ=255)                                                                                                                      cgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGt             >  1:743069/1‑139 (MQ=255)                                                                                                                                  cTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCg  <  2:105948/138‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       CTGTTAATACGTCTGCGCGATGCGGATTTTCAACACCAATCCACGCTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATATGTTATCTCCGCGTCATCAGAAGAACAGTGACGGATCGCCCGCCCGTTTGGTCAGGCG  >  NZ_CP009273/2550452‑2550718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑