| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 250,930 | A→G | I425T (ATC→ACC) | pepD ← | cytosol nonspecific dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 250,930 | 0 | A | G | 100.0% | 120.6 / NA | 32 | I425T (ATC→ACC) | pepD | cytosol nonspecific dipeptidase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/13); total (19/13) | |||||||||||
TGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTT > NZ_CP009273/250795‑251053 | tGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGgtgt > 1:597434/1‑139 (MQ=255) aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGtt < 2:111155/138‑1 (MQ=255) cGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCg < 2:576474/138‑1 (MQ=255) cGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGt > 2:191956/1‑139 (MQ=255) tCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCttgt > 1:839640/1‑139 (MQ=255) tgtgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGaa < 1:62281/139‑1 (MQ=255) agagTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCa < 1:426728/139‑1 (MQ=255) tgtgGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTa > 1:416811/1‑139 (MQ=255) tgGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTAc > 1:531352/1‑138 (MQ=255) tgGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTAc > 2:544627/1‑138 (MQ=255) tGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCg < 1:28816/96‑1 (MQ=255) tGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCg > 2:28816/1‑96 (MQ=255) tGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCAt > 1:257519/1‑139 (MQ=255) tGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCAt < 1:191956/139‑1 (MQ=255) gCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGgag > 2:10793/1‑138 (MQ=255) cccGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGaa > 1:703133/1‑139 (MQ=255) tGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTg < 2:839640/139‑1 (MQ=255) gATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTg < 2:564042/79‑1 (MQ=255) gATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTg > 1:564042/1‑79 (MQ=255) cacaTTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTg > 1:777674/1‑106 (MQ=255) cacaTTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTg < 2:777674/106‑1 (MQ=255) aTTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCa > 1:806144/1‑65 (MQ=255) aTTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCa < 2:806144/65‑1 (MQ=255) aGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCAc > 2:749446/1‑77 (MQ=255) aGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCAc < 1:749446/77‑1 (MQ=255) gCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCgccagc > 2:139816/1‑138 (MQ=255) cgTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCAc > 1:739130/1‑70 (MQ=255) cgTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCAc < 2:739130/70‑1 (MQ=255) cgTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGgag < 1:60859/75‑1 (MQ=255) cgTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGgag > 2:60859/1‑75 (MQ=255) cgTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGtt > 2:525260/1‑138 (MQ=255) cgTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGtt > 2:137099/1‑138 (MQ=255) cgTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGtt > 1:714548/1‑138 (MQ=255) | TGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTT > NZ_CP009273/250795‑251053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |