| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,637,057 | G→A | L196F (CTT→TTT) | rlmN ← | bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,637,057 | 0 | G | A | 95.0% | 138.5 / ‑0.8 | 40 | L196F (CTT→TTT) | rlmN | bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (20/18); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01 | |||||||||||
TGCAGGGAAATTGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGC > NZ_CP009273/2636939‑2637184 | tGCAGGGAAATTGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGaa < 1:141297/139‑1 (MQ=255)tGCAGGGAAATTGCCAGTGCAACGTCGATCATATCGCCCAATTTATCCAGCGCCGGAACTACGCCGGAAGTGGGAAGCGTGTCGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGaa < 2:472278/139‑1 (MQ=255) aTTGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACgttgt < 1:493774/139‑1 (MQ=255) ttGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACgttgtt > 1:427312/1‑139 (MQ=255) tGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACgttgt < 2:248264/137‑1 (MQ=255) tGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACgttgt > 1:248264/1‑137 (MQ=255) tGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGGACGACGTTGTTCAGGtt > 1:357306/1‑138 (MQ=255) gATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGc < 1:713364/139‑1 (MQ=255) aTCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCTACGTTTAGACAGGCCAAAACCGAAATCATCACACATGATTTCCATCGCCGGAACGACGGTGTTCAGGTTGAGCCGCGGCt > 2:287720/1‑139 (MQ=255) atCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCgcc > 1:383969/1‑138 (MQ=255) tCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCgccca < 1:700919/139‑1 (MQ=255) cGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCgcccat > 2:91781/1‑139 (MQ=255) aGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCgcccatgccca < 2:255587/139‑1 (MQ=255) gTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCgcccatgcccat > 2:718476/1‑139 (MQ=255) aTCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCcatcat < 1:741455/138‑1 (MQ=255) tCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCAtcatca < 1:126394/139‑1 (MQ=255) cgcCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACg > 2:618930/1‑139 (MQ=255) acacCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCg > 2:442893/1‑139 (MQ=255) cGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACg < 1:634701/139‑1 (MQ=255) gAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACt < 2:720030/120‑1 (MQ=255) gAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACt > 1:720030/1‑120 (MQ=255) gAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCt > 2:555724/1‑139 (MQ=255) gAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCgg > 1:136831/1‑139 (MQ=255) ggCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGCCGTTGTTCAGGTTGCGCAGCGGCTCGCCCCTGCCCATCATCACTACGTTAGTGATCGGCCGCTGACCGGTGACTTTTGCTGCGCCGACGCtttt > 2:297311/1‑139 (MQ=255) ggCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGAtttt > 2:240234/1‑139 (MQ=255) ggCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGAtttt > 1:661619/1‑139 (MQ=255) ggCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGAtttt > 1:145258/1‑139 (MQ=255) gCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCgccca < 1:614455/74‑1 (MQ=255) gCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCgccca > 2:614455/1‑74 (MQ=255) gCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTc > 2:120401/1‑139 (MQ=255) gCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTc > 1:133828/1‑139 (MQ=255) gCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTc > 1:193797/1‑139 (MQ=255) ccAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCt < 2:467490/106‑1 (MQ=255) ccAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCt > 1:467490/1‑106 (MQ=255) aaaaCCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgcc < 1:70211/139‑1 (MQ=255) aaaCCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgccg < 2:661619/139‑1 (MQ=255) aaaCCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTcgccg < 2:145258/139‑1 (MQ=255) aaaCCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTGGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGATGACCGGTGACGTTTGCGGCGCCGACGATTTTcgccg < 1:297311/139‑1 (MQ=255) gAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTCCGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGc < 2:858001/139‑1 (MQ=255) gAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGc < 1:555724/139‑1 (MQ=255) | TGCAGGGAAATTGCCAGTGCAACGTCGATCATATCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATCGGACGCTGACCGGTGACTTTTGCTGCGCCGACGATTTTCGCCGCACGC > NZ_CP009273/2636939‑2637184 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |