Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,644,099	T→G	A516A (GCA→GCC)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,644,099	0	T	G	100.0%	137.5 / NA	39	A516A (GCA→GCC)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/20);  total (19/20)

AACTGAACTTCATCCAGCGTGCGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGA  >  NZ_CP009273/2643965‑2644231                                                                                                                                       |                                                                                                                                     aaCTGAACTTCATCCAGCGTGCGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCa                                                                                                                                   <  1:377968/138‑1 (MQ=255) aaCTGAACTTCATCCAGCGTGCGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCAc                                                                                                                                  <  2:83910/139‑1 (MQ=255)      aaCTTCATCCAGCGTGCGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATa                                                                                                                              <  1:439224/138‑1 (MQ=255)           cATCCAGCGTGCGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAgg                                                                                                                          <  2:726757/137‑1 (MQ=255)           cATCCAGCGTGCGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGgt                                                                                                                         <  1:103955/138‑1 (MQ=255)            aTCCAGCGTGCGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGgtag                                                                                                                       <  2:377593/139‑1 (MQ=255)                aGCGTGCGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTAcc                                                                                                                   >  2:814187/1‑139 (MQ=255)                      cGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCccacca                                                                                                             <  1:299873/139‑1 (MQ=255)                                                    aaTTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGt                                                                                 >  2:713967/1‑137 (MQ=255)                                                    aaTTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGt                                                                                 >  2:272402/1‑137 (MQ=255)                                                         aaaaCCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTCGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCgg                                                                           <  2:117501/138‑1 (MQ=255)                                                           aaCCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGt                                                                        >  2:824571/1‑139 (MQ=255)                                                                        tGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGgtagta                                                                                                                     <  1:334283/81‑1 (MQ=255)                                                                        tGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGgtagta                                                                                                                     >  2:334283/1‑81 (MQ=255)                                                                          acacAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGt                                                            >  1:599646/1‑136 (MQ=255)                                                                          acacAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCa                                                          <  1:476991/138‑1 (MQ=255)                                                                               gCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCa                                                    <  1:824571/139‑1 (MQ=255)                                                                                cTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAgg                                                                                                                          <  1:461810/68‑1 (MQ=255)                                                                                cTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAgg                                                                                                                          >  2:461810/1‑68 (MQ=255)                                                                                cTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAg                                                   >  1:820284/1‑139 (MQ=255)                                                                                cTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAg                                                   >  1:284977/1‑139 (MQ=255)                                                                                cTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAg                                                   >  1:386683/1‑139 (MQ=255)                                                                                cTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAg                                                   >  1:760437/1‑139 (MQ=255)                                                                                          tGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATgcgc                                         >  1:693414/1‑139 (MQ=255)                                                                                            ggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGc                                                                                                                                    >  1:312073/1‑46 (MQ=255)                                                                                            ggCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGc                                                                                                                                    <  2:312073/46‑1 (MQ=255)                                                                                                    aaaaGTTGCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATa                                >  1:514784/1‑138 (MQ=255)                                                                                                           gCCCTTGCAAAGTATTACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCtt                         <  2:284977/138‑1 (MQ=255)                                                                                                                         ttACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATccc          <  1:659240/139‑1 (MQ=255)                                                                                                                         ttACCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATccc          <  1:90342/139‑1 (MQ=255)                                                                                                                           aCCATTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCca         >  2:835458/1‑138 (MQ=255)                                                                                                                              aTTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATc     <  1:445457/139‑1 (MQ=255)                                                                                                                              aTTAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATc     <  1:742194/139‑1 (MQ=255)                                                                                                                               ttAGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATcc    >  2:656884/1‑139 (MQ=255)                                                                                                                                 aGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTc                                                           <  2:289315/82‑1 (MQ=255)                                                                                                                                 aGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTc                                                           >  1:289315/1‑82 (MQ=255)                                                                                                                                 aGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATc            >  1:586901/1‑129 (MQ=255)                                                                                                                                 aGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATc            <  2:586901/129‑1 (MQ=255)                                                                                                                                 aGCAGGGGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGa  >  2:78787/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                     AACTGAACTTCATCCAGCGTGCGGGAAAGATTTTCGGCAGCTATATCGCCGAATTCAAAACCAGGTAAGGCTGACACAGCTTCACGCAGTGGGCGCAGGAAAAGTTGCCCTTGCAAAGTATTACCATTAGCAGGTGCACCATACAGGTAGTACCCCACCACGGAGAATTTCACTTCATCTTTCGGCGTTAGCGGGGTTTTCTCACCGGTCAGATTCAGCGCCATGCGCTCTGGCATAAAATCTTCGACGTGGAAATCCCACATCCGA  >  NZ_CP009273/2643965‑2644231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑