| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,664,311 | T→G | G15G (GGT→GGG) | hcaC → | bifunctional 3‑phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,664,311 | 0 | T | G | 83.7% | 129.2 / 21.3 | 49 | G15G (GGT→GGG) | hcaC | bifunctional 3‑phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base T (4/4); new base G (19/22); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
CCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGTGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGTCAGAAGGGTATCTGGAAGATGACG > NZ_CP009273/2664174‑2664438 | ccGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGTg < 1:48066/139‑1 (MQ=255) tCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGTGAgg < 2:602096/139‑1 (MQ=255) gACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGAt > 2:865421/1‑139 (MQ=255) aCAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATc < 1:467206/139‑1 (MQ=255) gcgcTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCt > 2:161906/1‑139 (MQ=255) gcgcTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCt < 1:125226/139‑1 (MQ=255) gcgcTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCt > 1:424004/1‑139 (MQ=255) gCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGcc < 2:526871/137‑1 (MQ=255) gATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCt > 1:365225/1‑139 (MQ=255) aaGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTggcggc < 1:422587/139‑1 (MQ=255) aGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTggcggc > 1:633390/1‑138 (MQ=255) aGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGgcggcg > 2:266496/1‑139 (MQ=255) gCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAgg > 1:237104/1‑87 (MQ=255) gCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAgg < 2:237104/87‑1 (MQ=255) gCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTggcggc > 1:848288/1‑137 (MQ=255) aCCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGa < 2:224084/138‑1 (MQ=255) cTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCg > 1:237198/1‑139 (MQ=255) aGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGtt < 1:865421/138‑1 (MQ=255) tACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGc > 2:68177/1‑139 (MQ=255) cTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCa > 1:36643/1‑139 (MQ=255) ttCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGTGAGGCTCTCCGGATCGATACCTCGcc < 1:39604/76‑1 (MQ=255) ttCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGTGAGGCTCTCCGGATCGATACCTCGcc > 2:39604/1‑76 (MQ=255) ttCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATgg > 2:399967/1‑139 (MQ=255) tCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATgg > 1:165178/1‑138 (MQ=255) tgatgaATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTaa > 1:151056/1‑139 (MQ=255) atgaATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGTGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATg > 2:727142/1‑139 (MQ=255) atgaATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTa < 1:68177/136‑1 (MQ=255) tCGAATTTATTCGTGTCCCGTTGCGGATGTGCCGTAGGGGGACGCTCTCCGGATCGATACCCCGCCCGTCATCGCCCTGATCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGGAATGCGTc > 2:507392/1‑138 (MQ=255) tCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGTGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTc > 2:367145/1‑138 (MQ=255) tCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTc > 2:26639/1‑138 (MQ=255) tCGAATTTATGCGTGTCCCGTTGCGGATGTCCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGTCATGGGAATGCGTc > 1:292707/1‑138 (MQ=255) gAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCaa < 1:826266/138‑1 (MQ=255) gAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCaa < 1:361908/138‑1 (MQ=255) aTTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGt < 2:365225/139‑1 (MQ=255) tttATGCGTGTCCCGTTGCGGATGTGCCGGAGGGTGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGt > 1:190333/1‑138 (MQ=255) tGCGTGTCCCGTTGCGGATGTGCCGGAGGGTGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGTCAGaa < 1:584806/139‑1 (MQ=255) tGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGTCAGaa < 2:424004/139‑1 (MQ=255) tGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGTCAGa < 1:26639/138‑1 (MQ=255) tGCGTGTCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGTCAGa < 1:399967/138‑1 (MQ=255) gtCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGTCAGAAGGGTa > 2:277058/1‑139 (MQ=255) tCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGcc < 2:245509/61‑1 (MQ=255) tCCCGTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGcc > 1:245509/1‑61 (MQ=255) gTTGCGGATGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGTCAGAAGGGTATCTgg < 2:370259/139‑1 (MQ=255) aTGTGCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGTCAGAAGGGTATCTGGAAGATGa < 1:277058/139‑1 (MQ=255) tgCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCGATGTCAGAAGGGTATCTGGAAGATGACg < 2:616511/138‑1 (MQ=255) tgCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGTCAGAAGGGTATCTGGAAGATGACg < 1:61322/138‑1 (MQ=255) tgCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGTCAGAAGGGTATCTGGAAGATGACg < 2:614599/138‑1 (MQ=255) tgCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGTCAGAAGGGTATCTGGAAGATGACg < 2:848288/138‑1 (MQ=255) tgCCGGAGGGGGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGTCAGAAGGGTATCTGGAAGATGACg < 2:257472/138‑1 (MQ=255) | CCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGGTGAGGCTCTCCGGATCGATACCTCGCCCGTCATCGCCCTGTTCAACGTTGGCGGCGAGTTTTATGCCATTAACGATCGTTGCAGCCATGGTAATGCGTCAATGTCAGAAGGGTATCTGGAAGATGACG > NZ_CP009273/2664174‑2664438 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |