Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,673,427 T→G N893T (AAC→ACC)  yphG ← DUF5107 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,673,4270TG80.7% 79.4 / 14.5 31N893T (AAC→ACC) yphGDUF5107 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (5/1);  new base G (12/13);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

AACCAGATGTCGTTATCAGTTTGCCCCGGTAAACGGCCTTCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCA  >  NZ_CP009273/2673289‑2673556
                                                                                                                                          |                                                                                                                                 
aaCCAGATGTCGTTATCAGTTTGCCCCGGTAAACGGCCTTCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGt                                                                                                                                   <  1:406300/139‑1 (MQ=255)
  ccAGATGTCGTTATCAGTTTGCCCCGGTAAACGGCCTTCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTg                                                                                                                                 <  2:464339/139‑1 (MQ=255)
  ccAGATGTCGTTATCAGTTTGCCCCGGTAAACGGCCTTCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTg                                                                                                                                 <  1:289515/139‑1 (MQ=255)
             tATCAGTTTGCCCCGGTAAACGGCCTTCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAact                                                                                                                       >  2:173414/1‑138 (MQ=255)
                     tGCCCCGGTAAACGGCCTTCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGa                                                                                                              >  1:267682/1‑139 (MQ=255)
                       ccccGGTAAACGGCCTTCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGAcc                                                                                                            >  2:748365/1‑139 (MQ=255)
                                  ggCCTTCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTcccc                                                                                                 <  1:705864/139‑1 (MQ=255)
                                       tCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCtt                                                                                            >  2:806655/1‑139 (MQ=255)
                                             aaaTTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCa                                                                                        <  1:237066/137‑1 (MQ=255)
                                                     cGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAAt                                                                              >  1:86879/1‑139 (MQ=255)
                                                           aTGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTgcgc                                                                        <  1:147977/139‑1 (MQ=255)
                                                           aTGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTgcg                                                                         <  1:806655/138‑1 (MQ=255)
                                                                 cgcgGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCTACGGGTGGAATTTGCGCGTGGc                                                                   >  1:330861/1‑138 (MQ=255)
                                                                 cgcgGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGc                                                                   >  2:846427/1‑138 (MQ=255)
                                                                      cATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAAt                                                             >  1:406146/1‑139 (MQ=255)
                                                                           aGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTccg                                                        <  2:266763/139‑1 (MQ=255)
                                                                                ttCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGAATGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCt                                                   >  1:521116/1‑139 (MQ=255)
                                                                                  cGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTgg                                                                    >  1:712618/1‑120 (MQ=255)
                                                                                  cGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTgg                                                                    <  2:712618/120‑1 (MQ=255)
                                                                                     aGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTc                                              >  1:231385/1‑139 (MQ=255)
                                                                                                 gCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGt                                  >  1:355065/1‑139 (MQ=255)
                                                                                                    gTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGt                                  >  1:657243/1‑136 (MQ=255)
                                                                                                    gTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGt                                  >  2:810027/1‑136 (MQ=255)
                                                                                                     tCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGt                                                                                                                                   <  2:767437/38‑1 (MQ=255)
                                                                                                     tCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGt                                                                                                                                   >  1:767437/1‑38 (MQ=255)
                                                                                                     tCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTgag                               <  2:355065/138‑1 (MQ=255)
                                                                                                     tCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCttcccttccc                                                                                                  >  2:539050/1‑71 (MQ=255)
                                                                                                     tCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCttcccttccc                                                                                                  <  1:539050/71‑1 (MQ=255)
                                                                                                         gCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGcc                          <  1:789584/139‑1 (MQ=255)
                                                                                                         gCATCAAGATGCTGCCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGcc                          <  1:688724/139‑1 (MQ=255)
                                                                                                                    ctgcCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAg                <  2:577564/138‑1 (MQ=255)
                                                                                                                    ctgcCAGGCGCGTAATAACTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAg                <  1:810027/138‑1 (MQ=255)
                                                                                                                          ggCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCt         >  1:218369/1‑139 (MQ=255)
                                                                                                                                 aataaCTGGGTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCa  >  1:754832/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                 
AACCAGATGTCGTTATCAGTTTGCCCCGGTAAACGGCCTTCGCTTAAATTCTCCGGATAATGCAGCGCGGCATGAAGCAGTTCGCAGGCCTGCTGCGGCTGTCTGGCATCAAGATGCTGCCAGGCGCGTAATAACTGGTTGAGGATAAACTGACTGGTGACCTTCCCTTCCCCGCCTTCCCACGGGTGGAATTTGCGCGTGGCGAGAATGTCCGCCGCTTTGTCTGCCTGACCCGTGAGATGCCACAAATTGAGCAGTTCTGCGGTCA  >  NZ_CP009273/2673289‑2673556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑