| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,682,977 | T→C | intergenic (‑112/+53) | qseG ← / ← qseE | two‑component system QseEF‑associated lipoprotein QseG/two component system sensor histidine kinase QseE/GlrK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,682,977 | 0 | T | C | 96.2% | 169.9 / ‑0.5 | 52 | intergenic (‑112/+53) | qseG/qseE | two‑component system QseEF‑associated lipoprotein QseG/two component system sensor histidine kinase QseE/GlrK |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (27/23); total (28/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01 | |||||||||||
ATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGT > NZ_CP009273/2682855‑2683105 | aTGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTg < 1:624355/139‑1 (MQ=255) atatTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACaaataaa < 1:152609/139‑1 (MQ=255) cACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAg < 2:306094/138‑1 (MQ=255) aCCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGcc > 2:357590/1‑139 (MQ=255) cAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTg < 1:610563/139‑1 (MQ=255) aCTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGaa > 2:400135/1‑116 (MQ=255) aCTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGaa < 1:400135/116‑1 (MQ=255) aCTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTga > 2:504628/1‑137 (MQ=255) aCTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTga > 2:623681/1‑137 (MQ=255) aaaGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTgatagga < 1:790824/138‑1 (MQ=255) aaGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTgataggata < 2:276669/139‑1 (MQ=255) tCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGt > 1:608855/1‑138 (MQ=255) tGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCat > 2:254947/1‑138 (MQ=255) cGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGcc < 2:495864/84‑1 (MQ=255) cGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGcc > 1:495864/1‑84 (MQ=255) cGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTAttt > 2:192863/1‑139 (MQ=255) gACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTcgac < 1:224249/139‑1 (MQ=255) gACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTcgac < 2:839319/139‑1 (MQ=255) tCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTg < 1:271125/96‑1 (MQ=255) tCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTg > 2:271125/1‑96 (MQ=255) tCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACaaataaaat < 1:137480/90‑1 (MQ=255) tCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACaaataaaat > 2:137480/1‑90 (MQ=255) gacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGaa > 1:660303/1‑66 (MQ=255) gacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGaa < 2:660303/66‑1 (MQ=255) gacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACaa > 1:809575/1‑73 (MQ=255) gacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACaa < 2:809575/73‑1 (MQ=255) gacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTga < 1:602695/87‑1 (MQ=255) gacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTga > 2:602695/1‑87 (MQ=255) gacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCCTTATTTCGTGTTTTTCGACGACGGTAATTCAATg > 2:343/1‑139 (MQ=255) gacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATg > 2:689707/1‑139 (MQ=255) gacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATg > 2:756927/1‑139 (MQ=255) gacaGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATg > 1:620934/1‑139 (MQ=255) gacaGCGCGGAGAAACCGGAGCGTACA‑ATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGaa > 2:691671/1‑65 (MQ=17) gacaGCGCGGAGAAACCGGAGCGTACA‑ATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGaa < 1:691671/65‑1 (MQ=17) gcgcGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCg < 2:258874/60‑1 (MQ=255) gcgcGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCg > 1:258874/1‑60 (MQ=255) cgcgGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGcc < 2:766669/51‑1 (MQ=255) cgcgGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGcc > 1:766669/1‑51 (MQ=255) cgcgGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTcgac > 2:92257/1‑119 (MQ=255) cgcgGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTcgac > 2:237381/1‑119 (MQ=255) cgcgGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTcgac < 1:92257/119‑1 (MQ=255) cgcgGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTcgac < 1:237381/119‑1 (MQ=255) ggAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGaaaca < 2:608855/139‑1 (MQ=255) gCGTACATATCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGccc > 2:406580/1‑139 (MQ=255) tCGTACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCtcgt < 1:406580/138‑1 (MQ=255) tACGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGAc > 1:32698/1‑139 (MQ=255) cGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTgataggata > 1:764296/1‑60 (MQ=255) cGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTgataggata < 2:764296/60‑1 (MQ=255) cGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGAc > 2:427981/1‑137 (MQ=255) cGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGAc > 2:33136/1‑137 (MQ=255) cGTGAAGATTTCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGAc > 1:557289/1‑137 (MQ=255) tttCGAGCACCGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGt < 1:858568/139‑1 (MQ=255) | ATGTGTCGCATATTCACCAGACTTAAAGCCTATCCCAGTGGGCGTAATTGTTGCAGACAGTCTGGACATGGACAGCGCGGAGAAACCGGAGCGTACATATCGTACGTGAAGATTTCGAGCACTGCCCGGGGCCGAACTGACAAATAAAATAGCCTGATAGGATAGGCTGTTAGCATTATTTCGTGTTTTTCGACGACGGTAATTCAATGCGGAAACAAACGTCTTGCCCGCTCTCGTCGACCAGATACAGT > NZ_CP009273/2682855‑2683105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |