Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	251,113	A→G	I364T (ATC→ACC)	pepD ←	cytosol nonspecific dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	251,113	0	A	G	100.0%	162.9 / NA	45	I364T (ATC→ACC)	pepD	cytosol nonspecific dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/23);  total (22/23)

CATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGATCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAAT  >  NZ_CP009273/250980‑251243                                                                                                                                      |                                                                                                                                   cATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAgg                                                                                                                               >  1:442633/1‑139 (MQ=255) cATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAgg                                                                                                                               <  2:397909/139‑1 (MQ=255) cATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAgg                                                                                                                               <  1:820015/139‑1 (MQ=255)   tCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAgg                                                                                                                               >  2:181941/1‑137 (MQ=255)     aCCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAgg                                                                                                                               >  2:787426/1‑135 (MQ=255)     aCCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAgg                                                                                                                               <  1:787426/135‑1 (MQ=255)     aCCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGt                                                                                                                           >  1:44223/1‑139 (MQ=255)         gagaATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAAt                                                                                                                       >  1:145195/1‑139 (MQ=255)                    tCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTAt                                                                                                            >  1:299247/1‑139 (MQ=255)                    tCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTAt                                                                                                            >  1:518676/1‑139 (MQ=255)                    tCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTAt                                                                                                            >  1:587450/1‑139 (MQ=255)                       ggCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTgt                                                                                                         <  2:587450/139‑1 (MQ=255)                                 cagGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGt                                                                                               <  2:518676/139‑1 (MQ=255)                                         gcgcCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCacac                                                                                       >  1:444721/1‑139 (MQ=255)                                          cgcCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACAcc                                                                                      <  1:107971/139‑1 (MQ=255)                                          cgcCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACAcc                                                                                      <  2:850502/139‑1 (MQ=255)                                           gcCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCg                                                                                     <  2:714548/139‑1 (MQ=255)                                            ccTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGa                                                                                    <  1:10793/139‑1 (MQ=255)                                             cTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGAc                                                                                   >  1:147761/1‑139 (MQ=255)                                              tttCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACg                                                                                  >  1:650348/1‑139 (MQ=255)                                               ttCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGt                                                                                 >  2:316684/1‑139 (MQ=255)                                                   cTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAg                                                                             >  1:686594/1‑139 (MQ=255)                                                      cGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAgg                                                                            <  2:630355/137‑1 (MQ=255)                                                                                  cGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCg                                              >  2:757681/1‑139 (MQ=255)                                                                                        cAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGtt                                         <  1:472446/138‑1 (MQ=255)                                                                                        cAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGtt                                         <  2:393549/138‑1 (MQ=255)                                                                                        cAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGtt                                         <  1:544627/138‑1 (MQ=255)                                                                                           cATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACg                                      >  2:351322/1‑138 (MQ=255)                                                                                            aTGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGaa                                    <  2:44223/139‑1 (MQ=255)                                                                                             tGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAAt                                   <  2:299247/139‑1 (MQ=255)                                                                                               cTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATca                                 <  1:537672/139‑1 (MQ=255)                                                                                                  accacGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATcaca                               <  2:145195/138‑1 (MQ=255)                                                                                                  accacGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATcaca                               <  2:686594/138‑1 (MQ=255)                                                                                                  accacGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATcac                                <  1:139816/137‑1 (MQ=255)                                                                                                         aGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACAt                                                <  2:382925/114‑1 (MQ=255)                                                                                                         aGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACAt                                                >  1:382925/1‑114 (MQ=255)                                                                                                              ttACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTgg                  >  2:70265/1‑139 (MQ=255)                                                                                                                     tGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCgggg                     >  1:718432/1‑129 (MQ=255)                                                                                                                     tGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCgggg                     <  2:718432/129‑1 (MQ=255)                                                                                                                     tGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTcag           >  2:105092/1‑139 (MQ=255)                                                                                                                       tCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTcag           >  2:66583/1‑137 (MQ=255)                                                                                                                       tCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTcag           <  2:531352/137‑1 (MQ=255)                                                                                                                         gATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGa       <  1:66583/139‑1 (MQ=255)                                                                                                                            cAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACg     >  1:596695/1‑138 (MQ=255)                                                                                                                               tGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAAt  >  2:442244/1‑138 (MQ=255)                                                                                                                                      |                                                                                                                                   CATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGATCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACATCGGAGTTACGAATCACACCGTTCGGGGTGGCGTTCAGCAGACGAAT  >  NZ_CP009273/250980‑251243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑