Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,745,770	A→G	E206G (GAG→GGG)	recN →	DNA repair protein RecN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,745,770	0	A	G	100.0%	155.2 / NA	44	E206G (GAG→GGG)	recN	DNA repair protein RecN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/21);  total (23/21)

CACGTTATCAGTTGTGGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGAGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTACACGGCTA  >  NZ_CP009273/2745635‑2745901                                                                                                                                        |                                                                                                                                    cACGTTATCAGTTGTGGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGtt                                                                                                                                  >  1:726018/1‑139 (MQ=255)              gtgGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcgac                                                                                                                     >  2:59819/1‑139 (MQ=255)              gtgGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcgac                                                                                                                     >  1:287453/1‑139 (MQ=255)               tgGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcgacg                                                                                                                    <  2:22729/139‑1 (MQ=255)               tgGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcgacg                                                                                                                    <  1:351260/139‑1 (MQ=255)               tgGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcgacg                                                                                                                    <  2:47590/139‑1 (MQ=255)                 gCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATcgacg                                                                                                                    >  1:9169/1‑137 (MQ=255)                 gCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGaa                                                                                                                  <  1:425416/139‑1 (MQ=255)                             ccGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGt                                                                                                      <  2:788239/139‑1 (MQ=255)                                       gcgcATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGaa                                                                                              >  1:203889/1‑137 (MQ=255)                                        cgcATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAg                                                                                           <  1:216476/139‑1 (MQ=255)                                        cgcATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAg                                                                                           >  2:81727/1‑139 (MQ=255)                                            tcatcaACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGt                                                                                       <  2:724810/139‑1 (MQ=255)                                                       tAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGac                                                                            >  2:404776/1‑139 (MQ=255)                                                         aGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGacca                                                                          >  1:662363/1‑139 (MQ=255)                                                          gTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCTCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTTGCGAACAGCGGTCAATTGCTGaccac                                                                         <  2:833423/139‑1 (MQ=255)                                                           tCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGaccac                                                                         >  1:176591/1‑138 (MQ=255)                                                                aaCGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCAccagc                                                                     >  2:447746/1‑137 (MQ=255)                                                                  cgcgCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGa                                                                 <  2:306049/139‑1 (MQ=255)                                                                    cgccgcCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAAt                                                               >  1:94979/1‑139 (MQ=255)                                                                    cgccgcCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAAt                                                               >  1:5070/1‑139 (MQ=255)                                                                                       ctgcAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACa                                                                                                           <  1:61512/76‑1 (MQ=255)                                                                                       ctgcAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACa                                                                                                           >  2:61512/1‑76 (MQ=255)                                                                                       ctgcAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGcc                                             >  2:780007/1‑138 (MQ=255)                                                                                        tgcAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGa                                           >  1:834203/1‑139 (MQ=255)                                                                                            aTACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGaccac                                                                         <  2:192599/105‑1 (MQ=255)                                                                                            aTACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGaccac                                                                         >  1:192599/1‑105 (MQ=255)                                                                                            aTACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGt                                       >  2:461010/1‑139 (MQ=255)                                                                                               ccAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGa                                     <  1:81727/138‑1 (MQ=255)                                                                                                 aaTTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGa                                  >  2:302744/1‑139 (MQ=255)                                                                                                  aTTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGAc                                 >  1:346700/1‑139 (MQ=255)                                                                                                       aaGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGaccac                                                                         <  1:516519/94‑1 (MQ=255)                                                                                                       aaGAACTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGaccac                                                                         >  2:516519/1‑94 (MQ=255)                                                                                                           aCTTAACGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTg                        >  1:409853/1‑139 (MQ=255)                                                                                                                 cGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGt                  <  2:9169/139‑1 (MQ=255)                                                                                                                 cGAATTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGt                  <  1:59819/139‑1 (MQ=255)                                                                                                                    aTTTAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAg               <  2:662363/139‑1 (MQ=255)                                                                                                                       tAATCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCtt            >  2:550103/1‑139 (MQ=255)                                                                                                                         aTCCGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGGCAGCTTTa          <  2:266006/139‑1 (MQ=255)                                                                                                                            cGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTacac       <  2:203889/139‑1 (MQ=255)                                                                                                                            cGCAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTacac       <  1:319523/139‑1 (MQ=255)                                                                                                                              cAGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTACACgg     >  2:602930/1‑139 (MQ=255)                                                                                                                               aGCCCGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTACACGGc    <  2:176591/139‑1 (MQ=255)                                                                                                                                 cccGGAGGGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTACACGGCTa  <  2:346700/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    CACGTTATCAGTTGTGGCATCAAAGCTGCCGTGACCTCGCGCATCATCAACAGTTAAGTCAGGAACGCGCCGCCCGTGCGGAACTGCTGCAATACCAATTAAAAGAACTTAACGAATTTAATCCGCAGCCCGGAGAGTTTGAACAAATCGACGAAGAGTACAAACGTCTGGCGAACAGCGGTCAATTGCTGACCACCAGCCAGAATGCATTGGCATTAATGGCCGACGGTGAAGACGCAAACCTGCAAAGTCAGCTTTACACGGCTA  >  NZ_CP009273/2745635‑2745901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑