Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,772,143 A→G S1315P (TCC→CCC)  ypjA ← adhesin‑like autotransporter YpjA/EhaD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,772,1430AG97.1% 112.9 / ‑3.6 34S1315P (TCC→CCC) ypjAadhesin‑like autotransporter YpjA/EhaD
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (15/18);  total (16/18)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

GTTCAGCTTCACGACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTACGGGTGTTA  >  NZ_CP009273/2772021‑2772276
                                                                                                                          |                                                                                                                                     
gTTCAGCTTCACGACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAgcg                                                                                                                       <  2:521538/139‑1 (MQ=255)
     gCTTCACGACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAg                                                                                                                  <  1:453481/139‑1 (MQ=255)
            gACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTa                                                                                                           <  2:525753/139‑1 (MQ=255)
                 cGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTcc                                                                                                      >  1:537030/1‑139 (MQ=255)
                 cGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTcc                                                                                                      >  2:228380/1‑139 (MQ=255)
                   tCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCtt                                                                                                    >  1:840597/1‑139 (MQ=255)
                     cAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTc                                                                                                   <  2:367854/138‑1 (MQ=255)
                         tAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGaa                                                                                              <  2:537030/139‑1 (MQ=255)
                          aGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGaa                                                                                              >  1:651287/1‑138 (MQ=255)
                           gAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAat                                                                                             <  2:840597/138‑1 (MQ=255)
                             aaCCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAatat                                                                                           >  1:180746/1‑138 (MQ=255)
                                  cTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTAc                                                                                     >  2:721907/1‑139 (MQ=255)
                                     tCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACgg                                                                                   <  1:338604/138‑1 (MQ=255)
                                               aaCTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATccc                                                                        >  2:84874/1‑139 (MQ=255)
                                               aaCTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATccc                                                                        >  1:411472/1‑139 (MQ=255)
                                                aCTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATcccc                                                                       <  1:481584/139‑1 (MQ=255)
                                                              ccAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGt                                                         >  1:725915/1‑139 (MQ=255)
                                                                    aaTAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGt                                                   >  1:405092/1‑139 (MQ=255)
                                                                        aCTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTg                                                <  2:693801/138‑1 (MQ=255)
                                                                         cTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCt                                              <  2:651287/139‑1 (MQ=255)
                                                                            cccACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCa                                            >  2:69721/1‑138 (MQ=255)
                                                                            cccACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCa                                            >  2:725831/1‑138 (MQ=255)
                                                                                      cATGTCCTCCGCGATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCAcc                                 <  1:814631/139‑1 (MQ=255)
                                                                                               cgcgATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGc                                                                                  <  1:825270/81‑1 (MQ=255)
                                                                                               cgcgATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGc                                                                                  >  2:825270/1‑81 (MQ=255)
                                                                                                  gATCAAAACCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCggtggt                     <  1:84874/139‑1 (MQ=255)
                                                                                                        aaCCGATATGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACatt               <  1:69721/139‑1 (MQ=255)
                                                                                                             atatGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTAc          <  2:481407/139‑1 (MQ=255)
                                                                                                             atatGTGAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTAc          <  2:725915/139‑1 (MQ=255)
                                                                                                              tatGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTACg         >  2:290600/1‑139 (MQ=255)
                                                                                                                tgtgAATGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCGTTACGGCTGTCGATCCCCACTGTCATTCCGTTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACAGTATTACgg        <  2:371097/138‑1 (MQ=255)
                                                                                                                     aTGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGaa                                                                                              <  1:66760/47‑1 (MQ=255)
                                                                                                                     aTGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGaa                                                                                              >  2:66760/1‑47 (MQ=255)
                                                                                                                     aTGGGGATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTACGGGTGTta  >  2:749948/1‑139 (MQ=255)
                                                                                                                          |                                                                                                                                     
GTTCAGCTTCACGACACCGTCCAGATAGAAACCACTTTCATGTTCCCAACTGGCATAGCCGCCCAGAGAATAACTGCCCACACTGCCATGTCCTCCGCGATCAAAACCGATATGTGAATGGGAATAGCCCATAAAAGCGCCTAGCGTGGTAATTCCTTCAGGAATATCATTACGGCTGTCGATCCCCACTGTCATTCCGGTCAGCGTCTGCTCAAACCCGGCACCCGCATCGGTGGTGACATTATTACGGGTGTTA  >  NZ_CP009273/2772021‑2772276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑