Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,808,251 T→G K516N (AAA→AAC)  gshA ← glutamate‑‑cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,808,2510TG90.2% 130.5 / 6.8 41K516N (AAA→AAC) gshAglutamate‑‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base G (19/18);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

TCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGA  >  NZ_CP009273/2808136‑2808380
                                                                                                                   |                                                                                                                                 
tcttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCaaa                                                                                                               <  2:2694/136‑1 (MQ=255)
tcttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCaaa                                                                                                               <  1:709233/136‑1 (MQ=255)
tcttcCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAAc                                                                                                              <  1:37392/137‑1 (MQ=255)
    cccAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGtt                                                                                                          <  2:826583/137‑1 (MQ=255)
      cAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCgg                                                                                                       >  2:730112/1‑138 (MQ=255)
             gTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCg                                                                                               >  1:495513/1‑139 (MQ=255)
              tCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCgg                                                                                              >  1:410512/1‑139 (MQ=255)
              tCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCgg                                                                                              >  1:653921/1‑139 (MQ=255)
                  aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTc                                                                                          >  1:856787/1‑139 (MQ=255)
                  aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGc                                                                                             <  1:18758/136‑1 (MQ=255)
                  aGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCtt                                                                                           <  2:170991/138‑1 (MQ=255)
                        tGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTc                                                                                    >  1:780194/1‑139 (MQ=255)
                              aCGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCctgct                                                                               >  2:466693/1‑138 (MQ=255)
                              aCGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCctgct                                                                               >  1:689519/1‑138 (MQ=255)
                              aCGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCctgct                                                                               >  1:143729/1‑138 (MQ=255)
                               cgcgCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGa                                                                             <  2:484451/139‑1 (MQ=255)
                                   cATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGc                                                                         <  2:352965/139‑1 (MQ=255)
                                              atcatcCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGcc                                                              >  1:712748/1‑139 (MQ=255)
                                                   cccTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTcgcg                                                         >  2:119975/1‑139 (MQ=255)
                                                    ccTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTcgcg                                                         <  2:679824/138‑1 (MQ=255)
                                                        aaTTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCgg                                                    >  1:598669/1‑139 (MQ=255)
                                                                  tGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCaaa                                                                                                               <  1:757572/70‑1 (MQ=255)
                                                                  tGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCaaa                                                                                                               >  2:757572/1‑70 (MQ=255)
                                                                            gCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCgcgc                                <  2:850783/139‑1 (MQ=255)
                                                                                   aCGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAAttt                         >  1:421748/1‑139 (MQ=255)
                                                                                          gCCTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCgg                  >  1:806606/1‑139 (MQ=255)
                                                                                           ccTTTTTCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCaaa                                                                                                               >  1:183786/1‑45 (MQ=255)
                                                                                           ccTTTTTCTTTTCTGCCAGGCGTGGTTTTCCAGCCACACCGCaaa                                                                                                               <  2:183786/45‑1 (MQ=38)
                                                                                            cTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGct                <  2:736527/139‑1 (MQ=255)
                                                                                             tttttCTTTTCTGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGctc               >  2:685022/1‑139 (MQ=255)
                                                                                               tttcttttctGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCtt             >  2:384209/1‑139 (MQ=255)
                                                                                               tttcttttctGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCtt             >  1:261595/1‑139 (MQ=255)
                                                                                                  cttttctGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCa           <  1:670465/138‑1 (MQ=255)
                                                                                                  cttttctGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCa           <  1:466693/138‑1 (MQ=255)
                                                                                                    tttctGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACg         >  2:281921/1‑138 (MQ=255)
                                                                                                     ttctGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACg         <  2:806606/137‑1 (MQ=255)
                                                                                                      tctGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACgca       <  2:717563/138‑1 (MQ=255)
                                                                                                       ctGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACgcag      <  2:143729/138‑1 (MQ=255)
                                                                                                       ctGTCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACgcag      <  1:385549/138‑1 (MQ=255)
                                                                                                          tCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACgcagca    <  1:176903/137‑1 (MQ=255)
                                                                                                          tCAGGCGTGGTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGa  <  2:821931/139‑1 (MQ=255)
                                                                                                                   |                                                                                                                                 
TCTTCCCAGGCGAGTCTGAGTATATGAAAGACGCGCATTTGTTATCATCATCCCTGAATTCAGAGATGAAATTTTGGCCACTCACGAGTGGCCTTTTTCTTTTCTGTCAGGCGTGTTTTTCCAGCCACACCGCAAACGGTTCGGTATCAGCGGCTTCCATTTCCTGCTGACGGCGTTCAGACGCCTCGCGCTCGGCTACAAAATCCTCTTCGCGCAGAATTTCCAGCGGCTCTTCACGCAGCAGA  >  NZ_CP009273/2808136‑2808380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑