Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,829,895	A→G	V297A (GTC→GCC)	hypF ←	carbamoyltransferase HypF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,829,895	0	A	G	100.0%	57.6 / NA	18	V297A (GTC→GCC)	hypF	carbamoyltransferase HypF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/10);  total (8/10)

CTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTATCCA  >  NZ_CP009273/2829759‑2829952                                                                                                                                         |                                                          cTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCt                                                         <  2:12175/139‑1 (MQ=255)    cAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCg                                                      >  1:649847/1‑139 (MQ=255)      aaTCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGtt                                                    <  2:791261/139‑1 (MQ=255)                        cGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGatat                                   <  1:697823/138‑1 (MQ=255)                                aTAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATcaca                            <  1:826918/137‑1 (MQ=255)                                aTAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATcaca                            <  2:649847/137‑1 (MQ=255)                                aTAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATcaca                            <  1:702744/137‑1 (MQ=255)                                     tggtggTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCt                       >  1:499762/1‑137 (MQ=255)                                                 gCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGGTATCATCACAAAGCTCAGGAACGTAtttt         >  1:138400/1‑139 (MQ=255)                                                 gCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGACCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTAtttt         >  1:88744/1‑139 (MQ=255)                                                  cTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTAttttt        >  1:722816/1‑139 (MQ=255)                                                  cTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTAttttt        >  1:495692/1‑139 (MQ=255)                                                     aGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTa      <  2:468719/138‑1 (MQ=255)                                                        ttGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTATcca  <  2:694015/139‑1 (MQ=255)                                                                                                    cAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTATcca  >  2:218140/1‑95 (MQ=255)                                                                                                    cAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTATcca  <  1:218140/95‑1 (MQ=255)                                                                                                      aaTGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCa                                             <  1:782840/50‑1 (MQ=255)                                                                                                      aaTGCTGGAGCGGGTTCGCAGGCAACATTACCCCGGCTTCGTTAAGGTCa                                             >  2:782840/1‑50 (MQ=255)                                                                                                                                         |                                                          CTGCAAATCCGCCAGCGCCTGTTCGTTGCTGATAGCTGGTGGTTTACCGCTCAGGTTGCCGGAGGTCATCACCAGCGGGCATTGCAGTTCCTGTAACAGCAAATGCTGGAGCGGGTTCGCAGGCAACATTACCCCGACTTCGTTAAGGTCAGGGGCGATATCATCACAAAGCTCAGGAACGTATTTTTTATCCA  >  NZ_CP009273/2829759‑2829952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑