Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,891,751	T→G	T343P (ACC→CCC)	ygcU ←	FAD‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,891,751	0	T	G	100.0%	115.2 / NA	33	T343P (ACC→CCC)	ygcU	FAD‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/16);  total (17/16)

GAATGACCGCCCAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGTTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCC  >  NZ_CP009273/2891618‑2891886                                                                                                                                      |                                                                                                                                        gAATGACCGCCCAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGgttt                                                                                                                                      >  2:544180/1‑137 (MQ=255)  aaTGACCGCCCAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTGCGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAg                                                                                                                                   <  2:157774/139‑1 (MQ=255)   aTGACCGCCCAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAgg                                                                                                                                  <  2:625189/139‑1 (MQ=255)   aTGACCGCCCAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAgg                                                                                                                                  <  2:39951/139‑1 (MQ=255)           ccAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTACATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGca                                                                                                                           >  1:38488/1‑138 (MQ=255)               cATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGt                                                                                                                      >  2:578620/1‑139 (MQ=255)                    tGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGc                                                                                                                 <  1:154017/139‑1 (MQ=255)                       tGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGc                                                                                                              >  2:570256/1‑139 (MQ=255)                              ggCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGGCACttt                                                                                                        <  1:172444/138‑1 (MQ=255)                                      ggAACTCAGTACGAATACGGTTAATAACGCTTTCGTACATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTcc                                                                                               <  2:38488/139‑1 (MQ=255)                                       gAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTccc                                                                                              <  2:274416/139‑1 (MQ=255)                                              gTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCa                                                                                       <  2:198484/139‑1 (MQ=255)                                               tACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAg                                                                                      >  2:830639/1‑139 (MQ=255)                                                 cGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAgg                                                                                     <  1:830639/138‑1 (MQ=255)                                                          ttAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAAcc                                                                           <  1:544180/139‑1 (MQ=255)                                                            aataaCGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAg                                                                         >  1:395581/1‑139 (MQ=255)                                                                  gCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTc                                                                    >  2:104844/1‑138 (MQ=255)                                                                      tCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGAt                                                                 >  2:223631/1‑137 (MQ=255)                                                                      tCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGAt                                                                 >  1:109766/1‑137 (MQ=255)                                                                           gATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTg                                                          <  1:512791/139‑1 (MQ=255)                                                                           gATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTg                                                          <  1:104844/139‑1 (MQ=255)                                                                                              cagcagCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCa                                         <  1:577356/137‑1 (MQ=255)                                                                                                 cagcCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCg                                    >  2:617851/1‑139 (MQ=255)                                                                                                    ccGGATACTTCGGTGGTAAAGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCggg                                  >  1:499230/1‑138 (MQ=255)                                                                                                                      aaGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTg                                                          <  2:258850/96‑1 (MQ=255)                                                                                                                      aaGCCCATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTg                                                          >  1:258850/1‑96 (MQ=255)                                                                                                                         cccATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCaa            >  2:831822/1‑139 (MQ=255)                                                                                                                          ccATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAAt           >  1:117921/1‑139 (MQ=255)                                                                                                                           cATGTTGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATc          >  2:675860/1‑139 (MQ=255)                                                                                                                               ttGCCGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCtt      <  1:223631/139‑1 (MQ=255)                                                                                                                                  ccGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCg                                                                                                   >  2:436968/1‑43 (MQ=255)                                                                                                                                  ccGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCg                                                                                                   <  1:436968/43‑1 (MQ=255)                                                                                                                                  ccGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGc   >  2:93229/1‑139 (MQ=255)                                                                                                                                   cGGGTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGcc  >  1:57268/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                        GAATGACCGCCCAGCATGGTGATGTCGTCGGCGTGCGGGAACTCAGTACGAATACGGTTAATAACGCTTTCGTAGATTTCGTGGATGCAGCTCCAGCAGCCGGATACTTCGGTGGTAAAGCCCATGTTGCCGGTTTTGAGGATCTGCACACGTTCGGCAGCCACTTTATCCGGTCCCCAGTTCAGGTTGTTAAACCAGGTTTCGATCAGCTTGCTGTCCACGCGCTGGCATTGCGGGTAGCGGGCAACGATTTCCGCAATCCCTTCGCC  >  NZ_CP009273/2891618‑2891886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑