| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,892,532 | A→C | G82G (GGT→GGG) | ygcU ← | FAD‑binding oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,892,532 | 0 | A | C | 89.8% | 159.1 / 7.7 | 49 | G82G (GGT→GGG) | ygcU | FAD‑binding oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (23/21); total (27/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01 | |||||||||||
CCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCA > NZ_CP009273/2892399‑2892649 | ccAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCgg > 1:261958/1‑138 (MQ=255)ccAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCgg > 2:583868/1‑138 (MQ=255)ccAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCgg > 2:340045/1‑138 (MQ=255) cAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGt < 2:668870/138‑1 (MQ=255) caccacATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGcac < 1:10672/139‑1 (MQ=255) caTTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGt < 2:813597/139‑1 (MQ=255) cgcCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAAtt > 2:166241/1‑139 (MQ=255) tCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGt > 1:2639/1‑115 (MQ=255) tCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGt < 2:2639/115‑1 (MQ=255) cATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCAt < 1:764600/137‑1 (MQ=255) tcaatatcaataTTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAAt > 1:710260/1‑139 (MQ=255) tcaatatTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGca < 1:825431/139‑1 (MQ=255) ccaatatTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGca < 1:647583/138‑1 (MQ=255) atatTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCacac < 1:583868/139‑1 (MQ=255) atatTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCacac < 1:415309/139‑1 (MQ=255) tatTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACg > 1:482906/1‑139 (MQ=255) ttAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACgg < 1:340045/138‑1 (MQ=255) aTGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACgg > 2:200180/1‑135 (MQ=255) aTGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACgg < 1:200180/135‑1 (MQ=255) tgatttgattCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATAc < 1:684073/139‑1 (MQ=255) tttgattCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTg < 2:160222/139‑1 (MQ=255) ttgattCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGc > 1:800927/1‑139 (MQ=255) ttgattCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGc > 1:296327/1‑139 (MQ=255) tGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGct > 1:72398/1‑132 (MQ=255) tGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGct < 2:72398/132‑1 (MQ=255) tGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTgg > 1:19320/1‑138 (MQ=255) ggCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGaa > 1:329511/1‑139 (MQ=255) ggCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGaa > 1:861528/1‑139 (MQ=255) gAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAAcc > 1:197524/1‑137 (MQ=255) aGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCg > 2:510924/1‑137 (MQ=255) aGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCg < 1:510924/137‑1 (MQ=255) tCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGcac > 1:223855/1‑107 (MQ=255) tCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGcac < 2:223855/107‑1 (MQ=255) ccaccGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGc > 1:108601/1‑139 (MQ=255) ccGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTg > 1:670910/1‑109 (MQ=255) ccGAGTTGTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTg < 2:670910/109‑1 (MQ=255) tCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCg > 1:680134/1‑139 (MQ=255) tCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCg > 2:709826/1‑139 (MQ=255) tACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCg > 1:69543/1‑40 (MQ=255) tACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCg < 2:69543/40‑1 (MQ=255) caacaGTTTCCAGCCCCCCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCaa < 1:338839/139‑1 (MQ=255) caacaGTTTCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCaa < 2:680134/139‑1 (MQ=255) tttCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtata < 2:800927/139‑1 (MQ=255) tttCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtat > 1:326462/1‑138 (MQ=255) tttCCAGCCCACCTTCGGTGGCGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGtat > 1:250991/1‑138 (MQ=255) tctatcccACCTTCGGTGGCGGAAGCACCGATACGCGGCACACCGTTAATTTTGTGCGCATTCATAAATTTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACAGCTGCTGGAATCGGCGGAGTATaaa > 2:438949/6‑139 (MQ=255) cccACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCa > 2:371014/1‑139 (MQ=255) cGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGAc > 2:760125/1‑94 (MQ=255) cGGAAGCCCCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGAc < 1:760125/94‑1 (MQ=255) aCCGGTACGCGGCACACCGTTAATTTTGTGCGCAt < 1:259490/35‑1 (MQ=255) aCCGGTACGCGGCACACCGTTAATTTTGTGCGCAt > 2:259490/1‑35 (MQ=255) | CCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCAATATCAATATTAATGATTTGATTCATGGCGGAGCCGTCGAGCACCACCGAGTTTTCTACAACAGTTTCCAGCCCACCTTCGGTGGCGGAAGCACCGGTACGCGGCACACCGTTAATTTTGTGCGCATTCATAAAATTCAGCACACGGGATACTTGCTCTGTGGAACCGAGTTTTACGACCGCTGCCGGAATCGGCAAAGTATAAATGCCA > NZ_CP009273/2892399‑2892649 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |