Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	253,379	T→C	G122G (GGT→GGC)	frsA →	esterase FrsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	253,379	0	T	C	100.0%	148.9 / NA	43	G122G (GGT→GGC)	frsA	esterase FrsA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/23);  total (20/23)

TGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTA  >  NZ_CP009273/253244‑253505                                                                                                                                        |                                                                                                                               tGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGgccgt                                                                                                                             >  1:189411/1‑139 (MQ=255)           aCCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCAc                                                                                                                          <  2:476022/132‑1 (MQ=255)           aCCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCAc                                                                                                                          >  1:476022/1‑132 (MQ=255)           aCCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTg                                                                                                                    >  1:726580/1‑138 (MQ=255)                  atgatTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGc                                                                                                            >  2:745166/1‑139 (MQ=255)                     atTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTa                                                                                                          <  2:91937/138‑1 (MQ=255)                     atTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTa                                                                                                          <  2:816112/138‑1 (MQ=255)                     atTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTa                                                                                                          <  2:265325/138‑1 (MQ=255)                     atTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTa                                                                                                          <  2:157043/138‑1 (MQ=255)                     atTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTa                                                                                                          <  1:515821/138‑1 (MQ=255)                     atTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTa                                                                                                          <  1:330827/138‑1 (MQ=255)                      tttGTACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGACGGTGTGGCAACAAAAAGCCTTTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACg                                                                                                        <  2:459016/139‑1 (MQ=255)                      tttATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACg                                                                                                        <  1:396441/139‑1 (MQ=255)                                  ggTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACAtt                                                                                            >  2:757895/1‑139 (MQ=255)                                         ggCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTgccgcc                                                                                      >  2:794789/1‑138 (MQ=255)                                              ccGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCt                                                                                <  1:704177/139‑1 (MQ=255)                                              ccGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCt                                                                                <  1:346230/139‑1 (MQ=255)                                                 tGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCAt                                                                             >  2:630208/1‑139 (MQ=255)                                                                     aGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTAACTGGCTGCATGCGGCTACGTTGTACACCATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGc                                                         <  2:388201/139‑1 (MQ=255)                                                                     aGTGGGCAACCCAGGCGATGTGTTGGCCACAAAAACCCCTGGCGGAACATACTTCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCGATTCTCAACTGAAAGGAGATGACCTGGc                                                         <  2:865271/139‑1 (MQ=255)                                                                                        ggTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCa                                                                                                                  <  2:206893/63‑1 (MQ=255)                                                                                        ggTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCa                                                                                                                  >  1:206893/1‑63 (MQ=255)                                                                                        ggTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAg                                                     >  2:619067/1‑124 (MQ=255)                                                                                        ggTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAg                                                     <  1:619067/124‑1 (MQ=255)                                                                                        ggTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTg                                      >  2:186825/1‑139 (MQ=255)                                                                                        ggTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTg                                      >  1:837363/1‑139 (MQ=255)                                                                                        ggTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTg                                      >  1:821077/1‑139 (MQ=255)                                                                                        ggTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTg                                      >  2:472313/1‑139 (MQ=255)                                                                                        ggTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCCATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTg                                      >  2:62705/1‑139 (MQ=255)                                                                                         gtgtGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGGACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGt                                     >  2:119976/1‑139 (MQ=255)                                                                                            tgGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCa                                   >  2:442188/1‑138 (MQ=255)                                                                                                aacaaAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcg                              <  1:442188/139‑1 (MQ=255)                                                                                                aacaaAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcg                              <  2:472288/139‑1 (MQ=255)                                                                                                aacaaAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcg                              <  1:472313/139‑1 (MQ=255)                                                                                                aacaaAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACcg                              <  2:632260/139‑1 (MQ=255)                                                                                                    aaaaGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCt                                         <  1:649416/124‑1 (MQ=255)                                                                                                    aaaaGCCTGTGCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCt                                         >  2:649416/1‑124 (MQ=255)                                                                                                            gtgCGGAAGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGaa                    >  1:543293/1‑137 (MQ=255)                                                                                                                  aaGACGATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGc            <  1:757895/139‑1 (MQ=255)                                                                                                                       gATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTg                                                                                           <  2:691638/55‑1 (MQ=255)                                                                                                                       gATCCGCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTg                                                                                           >  1:691638/1‑55 (MQ=255)                                                                                                                            gCAACTCAGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTa  >  1:161942/1‑139 (MQ=255)                                                                                                                                   aGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGGTc          >  2:90717/1‑124 (MQ=255)                                                                                                                                   aGTGGCCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTc          <  1:90717/124‑1 (MQ=255)                                                                                                                                        |                                                                                                                               TGCCGAACGTACCGACGATGATTTATACGATACGGTGATTGGCTACCGTGGCGGCAACTGGATTTATGAGTGGGCCACCCAGGCGATGGTGTGGCAACAAAAAGCCTGTGCGGAAGACGATCCGCAACTCAGTGGTCGTCACTGGCTGCATGCGGCTACGTTGTACAACATTGCCGCCTATCCTCATCTGAAAGGAGATGACCTGGCCGAGCAAGCGCAGGCTTTGTCAAACCGCGCCTATGAAGAGGCCGCTCAGCGTCTA  >  NZ_CP009273/253244‑253505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑