Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,931,836	A→G	D337G (GAT→GGT)	fucK →	L‑fuculokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,931,836	0	A	G	98.5%	222.8 / ‑3.8	65	D337G (GAT→GGT)	fucK	L‑fuculokinase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (28/36);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CAGGGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCA  >  NZ_CP009273/2931704‑2931964                                                                                                                                     |                                                                                                                                   caggGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCg                                                                                                                               >  2:437007/1‑138 (MQ=255) caggGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCg                                                                                                                               >  1:458404/1‑138 (MQ=255) caggGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGt                                                                                                                              >  2:478670/1‑139 (MQ=255)     gTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑aaaa                                                                                                                        <  1:352911/139‑1 (MQ=255)     gTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑aaa                                                                                                                         >  1:706618/1‑138 (MQ=255)      ttGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑aaaaa                                                                                                                       <  1:478670/139‑1 (MQ=255)         tataACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATg                                                                                                                     >  1:851438/1‑138 (MQ=255)         tataACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGT‑‑AAAAATg                                                                                                                     >  2:217129/1‑138 (MQ=255)           taACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCa                                                                                                                   <  2:549377/138‑1 (MQ=255)              cccAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAgtgt                                                                                                               >  1:523053/1‑139 (MQ=255)              cccAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAgtgt                                                                                                               >  1:451532/1‑139 (MQ=255)               ccAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGtgtg                                                                                                              <  1:148516/139‑1 (MQ=255)                cAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGa                                                                                                             >  1:319111/1‑139 (MQ=255)                   gTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGAtt                                                                                                           >  2:93754/1‑138 (MQ=255)                   gTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGAtt                                                                                                           >  1:101268/1‑138 (MQ=255)                        cAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAgtg                                                                                                                >  2:74837/1‑128 (MQ=255)                        cAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAgtg                                                                                                                <  1:74837/128‑1 (MQ=255)                        cAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGt                                                                                                     <  2:405956/139‑1 (MQ=255)                         aaTGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTc                                                                                                    >  1:422826/1‑139 (MQ=255)                             gctggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCgtgt                                                                                                >  1:104822/1‑139 (MQ=255)                             gctggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCgtgt                                                                                                >  2:863783/1‑139 (MQ=255)                             gctggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCgtgt                                                                                                >  2:445669/1‑139 (MQ=255)                             gctggcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCgtgt                                                                                                >  2:542300/1‑139 (MQ=255)                                 gcATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCa                                                                                              <  1:863783/137‑1 (MQ=255)                                    tCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACg                                                                                         <  2:523053/139‑1 (MQ=255)                                       ggCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACgctg                                                                                      >  2:546291/1‑139 (MQ=255)                                        gCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACgctgg                                                                                     <  1:129576/139‑1 (MQ=255)                                           tGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACgctggctg                                                                                  >  1:542846/1‑139 (MQ=255)                                             cTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGGGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCtggctggc                                                                                <  1:806760/139‑1 (MQ=255)                                                gAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCa                                                                               <  2:575230/137‑1 (MQ=255)                                                     ggTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGtgtg                                                                                                              >  1:230579/1‑101 (MQ=255)                                                     ggTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGtgtg                                                                                                              <  2:230579/101‑1 (MQ=255)                                                     ggTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGt                                                                         >  1:597959/1‑138 (MQ=255)                                                     ggTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTg                                                                        <  1:437007/139‑1 (MQ=255)                                                     ggTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTg                                                                        <  2:101268/139‑1 (MQ=255)                                                     ggTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTg                                                                        <  1:707336/139‑1 (MQ=255)                                                     ggTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTg                                                                        <  2:260788/139‑1 (MQ=255)                                                          gaAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACg                                                                     >  2:523958/1‑139 (MQ=255)                                                                       gACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCAcgc                                                        <  1:586318/137‑1 (MQ=255)                                                                        aCGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCggg                                                     <  1:698139/139‑1 (MQ=255)                                                                          ggCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCggggg                                                   <  1:445669/139‑1 (MQ=255)                                                                          ggCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCggggg                                                   <  2:319111/139‑1 (MQ=255)                                                                          ggCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCggggg                                                   <  1:93754/139‑1 (MQ=255)                                                                            cTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGTAAAAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCggggg                                                   <  1:523958/139‑1 (MQ=255)                                                                            cTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTg                                                                        >  2:149950/1‑116 (MQ=255)                                                                            cTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTg                                                                        <  1:149950/116‑1 (MQ=255)                                                                                      cTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCa                                                                                                                   <  1:491836/63‑1 (MQ=255)                                                                                      cTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCa                                                                                                                   >  2:491836/1‑63 (MQ=255)                                                                                      cTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATcgc                                       <  2:706618/139‑1 (MQ=255)                                                                                      cTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATcgc                                       >  1:338215/1‑139 (MQ=255)                                                                                         gCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACgctgg                                                                                     <  2:221057/90‑1 (MQ=255)                                                                                         gCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACgctgg                                                                                     >  1:221057/1‑90 (MQ=255)                                                                                         gCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATcgcg                                      <  2:450543/137‑1 (MQ=255)                                                                                         gCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATcgcg                                      <  2:370461/137‑1 (MQ=255)                                                                                                ttgattgaAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTg                              <  2:597959/138‑1 (MQ=255)                                                                                                   attgaAGAAGCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAg                          >  2:432373/1‑139 (MQ=255)                                                                                                          aagCTCGTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAAc                   >  2:824776/1‑139 (MQ=255)                                                                                                                gTCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTgcg               <  1:432373/137‑1 (MQ=255)                                                                                                                 tCTGATCGCGCCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCaa            >  1:848748/1‑139 (MQ=255)                                                                                                                         cgcCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAgcg    <  2:422826/139‑1 (MQ=255)                                                                                                                         cgcCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAgcg    <  1:326449/139‑1 (MQ=255)                                                                                                                         cgcCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAgcg    <  2:829683/139‑1 (MQ=255)                                                                                                                         cgcCTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAgcg    <  1:452216/139‑1 (MQ=255)                                                                                                                           ccTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCa  <  1:295826/139‑1 (MQ=255)                                                                                                                           ccTGGCGCGGGTGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCa  <  2:104822/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   CAGGGTTGTATAACCCAGGTATGCAATGGCTGGCATCCGGCGTGCTGGAATGGGTGAGAAAACTGTTCTGGACGGCTGAAACACCCTGGCAAATGTTGATTGAAGAAGCTCGTCTGATCGCGCCTGGCGCGGATGGCGT‑‑AAAAATGCAGTGTGATTTATTGTCGTGTCAGAACGCTGGCTGGCAAGGAGTGACGCTTAATACCACGCGGGGGCATTTCTATCGCGCGGCGCTGGAAGGGTTAACTGCGCAATTACAGCGCA  >  NZ_CP009273/2931704‑2931964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑