Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,959,152	A→G	F82S (TTC→TCC)	lgt ←	prolipoprotein diacylglyceryl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,959,152	0	A	G	100.0%	162.8 / NA	46	F82S (TTC→TCC)	lgt	prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/23);  total (23/23)

AGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATT  >  NZ_CP009273/2959024‑2959286                                                                                                                                 |                                                                                                                                       agaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTagaa                                                                                                                              <  2:66245/139‑1 (MQ=255) agaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTagaa                                                                                                                              <  2:677140/139‑1 (MQ=255) agaagGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTagaa                                                                                                                              <  1:576022/139‑1 (MQ=255)        aCGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAgaacagaaca                                                                                                                       <  1:401489/139‑1 (MQ=255)                              aTAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAgg                                                                                                  >  2:593994/1‑138 (MQ=255)                                      caccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTccc                                                                                                        >  2:558040/1‑124 (MQ=255)                                      caccacGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTccc                                                                                                        <  1:558040/124‑1 (MQ=255)                                          acGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGa                                                                                     <  2:578832/139‑1 (MQ=255)                                           cGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAgg                                                                                      >  1:29723/1‑137 (MQ=255)                                             aTAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGc                                                                                  <  1:775194/139‑1 (MQ=255)                                              tAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGcc                                                                                 >  1:340762/1‑139 (MQ=255)                                                aCGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCg                                                                               <  2:546410/139‑1 (MQ=255)                                                 cGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTa                                                                                                                                 >  1:112383/1‑88 (MQ=255)                                                 cGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTa                                                                                                                                 <  2:112383/88‑1 (MQ=255)                                                   ccAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCAt                                                                            <  2:340762/139‑1 (MQ=255)                                                   ccAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGAACGGCCACAAACTGCGGGGAATTGTAGAACAGAACAAAACAAATACGACCCCCGAGGTAGACGCCGAGGAAGCCCGCa                                                                             >  2:862268/1‑138 (MQ=255)                                                      aTCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAg                                                                                       >  2:857770/1‑125 (MQ=255)                                                      aTCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAg                                                                                       <  1:857770/125‑1 (MQ=255)                                                      aTCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATa                                                                           >  1:426258/1‑137 (MQ=255)                                                         aGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGt                                                                       <  1:76628/138‑1 (MQ=255)                                                           gccgccGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGaa                                                                                                <  1:724227/111‑1 (MQ=255)                                                           gccgccGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGaa                                                                                                >  2:724227/1‑111 (MQ=255)                                                           gccgccGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGCAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAg                                                                    >  2:92188/1‑139 (MQ=255)                                                                cgTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTagaacagaa                                                                                                                         >  1:238066/1‑81 (MQ=255)                                                                cgTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTagaacagaa                                                                                                                         <  2:238066/81‑1 (MQ=255)                                                                cgTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGaa                                                                                                >  2:492610/1‑106 (MQ=255)                                                                cgTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGaa                                                                                                <  1:492610/106‑1 (MQ=255)                                                                         aCATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCAtt                                                      >  2:822434/1‑139 (MQ=255)                                                                            tGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTg                                                                                                                                   >  2:742831/1‑59 (MQ=255)                                                                            tGCCGCCGTCCCAGACACGGAACAGACACAGCGGATCGGCCATAAACTGCGGGGAATTg                                                                                                                                   <  1:742831/59‑1 (MQ=255)                                                                                  cgTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATag                                                                          >  1:144424/1‑110 (MQ=255)                                                                                  cgTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATag                                                                          <  2:144424/110‑1 (MQ=255)                                                                                              ggAACAGATACAGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCg                                  >  1:62661/1‑138 (MQ=255)                                                                                               gAACAGATACAGCGGATCTGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGa                                <  2:781735/139‑1 (MQ=255)                                                                                                         aGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAg                                                                                       <  2:398629/74‑1 (MQ=255)                                                                                                         aGCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAg                                                                                       >  1:398629/1‑74 (MQ=255)                                                                                                          gCGGATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCcg                     >  1:159948/1‑139 (MQ=255)                                                                                                            ggATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCcgtc                   >  2:236754/1‑139 (MQ=255)                                                                                                             gATCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCcgtcg                  >  2:299108/1‑139 (MQ=255)                                                                                                               tCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCgtg                <  2:368119/139‑1 (MQ=255)                                                                                                               tCGGCCATAAACTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCgtg                <  2:62661/139‑1 (MQ=255)                                                                                                                         aCTGCGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTgccagcca      <  1:92188/139‑1 (MQ=255)                                                                                                                             cGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACt                                                           <  1:747817/82‑1 (MQ=255)                                                                                                                             cGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACt                                                           >  2:747817/1‑82 (MQ=255)                                                                                                                             cGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACAtt  >  2:262041/1‑139 (MQ=255)                                                                                                                             cGGGGAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACAtt  >  1:471057/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                       AGAAGGAACGTTTAGTACGGCGGGCGAAGATAATCATCACCACGATAACGCCAATCAGGCCGCCGTGGAAAGACATGCCGCCGTCCCAGACACGGAACAGATACAGCGGATCGGCCATAAACTGCGGGAAATTGTAGAACAGAACATAACCAATACGTCCCCCGAGGAAGACGCCGAGGAAGCCCGCATAGAGTAAGTTTTCAACTTCATTTTTGGTCCAGCCGCTGCCCGGACGATTCGCCCGTCGTGTTGCCAGCCACATT  >  NZ_CP009273/2959024‑2959286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑