Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,071,778	A→C	G147G (GGT→GGG)	cmtA ←	PTS mannitol transporter subunit IICB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,071,778	0	A	C	100.0%	202.8 / NA	56	G147G (GGT→GGG)	cmtA	PTS mannitol transporter subunit IICB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/29);  total (27/29)

TTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAAACCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCC  >  NZ_CP009273/3071647‑3071905                                                                                                                                    |                                                                                                                                ttGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCcagca                                                                                                                          <  2:109023/139‑1 (MQ=255) ttGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCcagca                                                                                                                          <  2:448025/139‑1 (MQ=255)   gAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGa                                                                                                                        >  2:715557/1‑139 (MQ=255)      gAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACa                                                                                                                      >  1:49972/1‑138 (MQ=255)           gCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAg                                                                                                                >  1:256322/1‑139 (MQ=255)           gCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAg                                                                                                                >  1:307513/1‑139 (MQ=255)           gCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAg                                                                                                                >  1:554271/1‑139 (MQ=255)              cTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCAt                                                                                                             <  1:298427/139‑1 (MQ=255)                 tCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATc                                                                                                            <  2:373133/137‑1 (MQ=255)                    ccGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCcgc                                                                                                       <  1:113973/139‑1 (MQ=255)                     cGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCcgcg                                                                                                      <  2:49972/139‑1 (MQ=255)                     cGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCcgcg                                                                                                      <  1:483396/139‑1 (MQ=255)                      ggTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGa                                                                                                     <  2:167333/139‑1 (MQ=255)                          cATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATAc                                                                                                  <  1:407271/138‑1 (MQ=255)                          cATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATAc                                                                                                  <  1:407251/138‑1 (MQ=255)                                  actgacAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATgag                                                                                          >  1:524719/1‑138 (MQ=255)                                      acaacaATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAg                                                                                       <  1:71417/137‑1 (MQ=255)                                      acaacaATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAg                                                                                       <  1:530768/137‑1 (MQ=255)                                      acaacaATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAg                                                                                       <  2:524719/137‑1 (MQ=255)                                             tGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGAt                                                                               >  2:283154/1‑138 (MQ=255)                                               gCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAAc                                                                            <  1:283154/139‑1 (MQ=255)                                                cAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATc                                                                                                            >  2:212343/1‑106 (MQ=255)                                                cAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATc                                                                                                            <  1:212343/106‑1 (MQ=255)                                                cAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAAc                                                                            >  2:863136/1‑138 (MQ=255)                                                           cATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCAtc                                                                        <  1:219299/131‑1 (MQ=255)                                                           cATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCAtc                                                                        >  2:219299/1‑131 (MQ=255)                                                             tGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCca                                                                                                                             <  1:767403/76‑1 (MQ=255)                                                             tGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCca                                                                                                                             >  2:767403/1‑76 (MQ=255)                                                             tGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCgg                                                              >  2:862152/1‑139 (MQ=255)                                                               tACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCgg                                                              >  2:400581/1‑137 (MQ=255)                                                               tACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGc                                                             <  2:193226/138‑1 (MQ=255)                                                                aCCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAg                                                           >  1:761597/1‑139 (MQ=255)                                                                     cgcCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATg                                                      >  2:579139/1‑139 (MQ=255)                                                                        cTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGt                                                   <  1:862152/139‑1 (MQ=255)                                                                                acTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAg                                           >  2:423999/1‑139 (MQ=255)                                                                                  tctTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTg                                         >  1:724946/1‑139 (MQ=255)                                                                                  tctTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTg                                         >  1:288852/1‑139 (MQ=255)                                                                                     ttGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCt                                      >  2:331411/1‑139 (MQ=255)                                                                                     ttGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCt                                      >  2:373926/1‑139 (MQ=255)                                                                                     ttGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCt                                      >  2:848820/1‑139 (MQ=255)                                                                                       gACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTa                                    >  2:656412/1‑139 (MQ=255)                                                                                          gAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCcg                                                                                                        <  2:659525/68‑1 (MQ=255)                                                                                          gAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCcg                                                                                                        >  1:659525/1‑68 (MQ=255)                                                                                            aaGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCcgc                                                                                                       >  2:456022/1‑67 (MQ=255)                                                                                            aaGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCcgc                                                                                                       <  1:456022/67‑1 (MQ=255)                                                                                             aGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACa                              <  2:307513/139‑1 (MQ=255)                                                                                                     cAATTAACACCGCCGGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTAt                      <  2:724946/139‑1 (MQ=255)                                                                                                              ccgccgGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAg                                                                                       <  1:616200/65‑1 (MQ=255)                                                                                                              ccgccgGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAg                                                                                       >  2:616200/1‑65 (MQ=255)                                                                                                                gccgGGCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCAc           >  1:507933/1‑139 (MQ=255)                                                                                                                    ggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAg       <  2:507933/139‑1 (MQ=255)                                                                                                                    ggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAg       <  1:848820/139‑1 (MQ=255)                                                                                                                    ggCCGATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAg       <  1:331411/139‑1 (MQ=255)                                                                                                                        gATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGc   <  1:39595/139‑1 (MQ=255)                                                                                                                        gATAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGc   <  1:400581/139‑1 (MQ=255)                                                                                                                         aTAACTTCAAACCCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGcc  >  1:623028/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                TTGAGGAAAAGCACTTTCGCCGGTTCATTGATGACTGACAACAATGGCAGATAACCCGCATGTACCAGCGCCTCAATACACTCTTTGACGAAAGTATTGGCAATTAACACCGCCGGGCCGATAACTTCAAAACCCAGCAGACAAAGGAGCATCCCCGCGATACCTAATGAGAAGTTATTGATAACCATCTCAAAACCGGCAGGTATGCGTTTTTCCAGTGCCTTATCGACATATTTTATGACCAACCCACCGAGCGGCC  >  NZ_CP009273/3071647‑3071905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑