Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,148,355	A→G	S56P (TCC→CCC)	yqhC ←	DNA‑binding transcriptional regulator YqhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,148,355	0	A	G	100.0%	89.3 / NA	26	S56P (TCC→CCC)	yqhC	DNA‑binding transcriptional regulator YqhC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/11);  total (15/11)

GACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTAT  >  NZ_CP009273/3148220‑3148477                                                                                                                                        |                                                                                                                           gaCGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGgaaa                                                                                                                         <  2:522766/139‑1 (MQ=255)    gCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAagag                                                                                                                      <  1:392886/139‑1 (MQ=255)      aaCCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGaa                                                                                                                    >  2:530384/1‑139 (MQ=255)           tGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTat                                                                                                                >  1:630262/1‑138 (MQ=255)               aGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGc                                                                                                           <  1:530384/139‑1 (MQ=255)                   gCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAgg                                                                                                       >  1:536929/1‑139 (MQ=255)                               tGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCAc                                                                                           <  1:257360/139‑1 (MQ=255)                                  cATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCgg                                                                                        >  1:98103/1‑139 (MQ=255)                                           cGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGc                                                                               >  2:738561/1‑139 (MQ=255)                                                   aacggcaacggcACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCg                                                                       <  2:371470/139‑1 (MQ=255)                                                      ggcaacggcaCCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGtct                                                                    <  1:601559/139‑1 (MQ=255)                                                                     agcagcagGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACaaa                                                      <  2:198079/138‑1 (MQ=255)                                                                            ggTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATc                                              <  1:283363/139‑1 (MQ=255)                                                                                   aTTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCaa                                        <  2:851428/138‑1 (MQ=255)                                                                                     tGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAg                                     >  2:571822/1‑139 (MQ=255)                                                                                        catcatAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGCAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTcc                                  >  2:649311/1‑139 (MQ=255)                                                                                        catcatAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTcc                                  >  2:32690/1‑139 (MQ=255)                                                                                                   aaCACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCaa                                        >  2:714688/1‑122 (MQ=255)                                                                                                   aaCACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCaa                                        <  1:714688/122‑1 (MQ=255)                                                                                                   aaCACGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTAt                       >  2:724832/1‑139 (MQ=255)                                                                                                     cacGCGTTCATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTAttt                     >  2:698789/1‑139 (MQ=255)                                                                                                             cATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTa                                                                                                 <  2:191156/55‑1 (MQ=255)                                                                                                             cATTGATATAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTa                                                                                                 >  1:191156/1‑55 (MQ=255)                                                                                                                  atatAACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTc         <  2:98103/138‑1 (MQ=255)                                                                                                                    ataACCGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGt      >  2:450561/1‑139 (MQ=255)                                                                                                                        ccGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTttat  >  1:326410/1‑139 (MQ=255)                                                                                                                        ccGATTTTATGCCCGGGAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTttat  >  1:204480/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                           GACGCAACCCTGCCAGCGGCACCTCTGACGTGGCATAGGTTTCGCACTCAAACGGCAACGGCACCGTCAGCAGCAGGTATTCATTGGCATCATAACGAAACACGCGTTCATTGATATAACCGATTTTATGCCCGGAAAAGAGAATTATGATGCCAGGCTCGTACATCACCGGTGTACGTGCGAAAGGCGTCTCGCCATACAACAAACGCACATCGGGCAACAGTCCTGACAAACTATTTTCTTTATTTTTCAGTTTAT  >  NZ_CP009273/3148220‑3148477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑