| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,155,922 | A→G | V36A (GTT→GCT) | ftsP ← | cell division protein FtsP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,155,922 | 0 | A | G | 93.8% | 196.2 / 4.5 | 65 | V36A (GTT→GCT) | ftsP | cell division protein FtsP |
| Reads supporting (aligned to +/- strand): ref base A (2/2); new base G (30/31); total (32/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
GTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGCTA > NZ_CP009273/3155796‑3156052 | gTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGt < 2:81167/138‑1 (MQ=255) gTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCg > 1:480393/1‑139 (MQ=255) cGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTg > 1:18173/1‑139 (MQ=255) aTTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTg < 2:252343/139‑1 (MQ=255) ttGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTg > 2:352136/1‑125 (MQ=255) ttGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTg < 1:352136/125‑1 (MQ=255) ttGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTgg > 2:288303/1‑139 (MQ=255) ttGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGCACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTgg > 1:645289/1‑139 (MQ=255) aTTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGcc < 2:517905/138‑1 (MQ=255) ccGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAAc < 2:374031/139‑1 (MQ=255) aCGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGt < 2:683567/101‑1 (MQ=255) aCGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGt > 1:683567/1‑101 (MQ=255) cgcgcgTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTgcg < 2:487922/118‑1 (MQ=255) cgcgcgTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTgcg > 1:487922/1‑118 (MQ=255) cgcgcgTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAAc > 1:230179/1‑135 (MQ=255) cgcgcgTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAAc < 2:230179/135‑1 (MQ=255) cgcgcgTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAgcg > 2:448194/1‑139 (MQ=255) cgcgTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGcc < 2:38444/139‑1 (MQ=255) gcgTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCt < 2:764570/139‑1 (MQ=255) ccTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGcaca > 2:359868/1‑137 (MQ=255) ggCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTg > 2:855949/1‑139 (MQ=255) aaTGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATcc > 2:663511/1‑139 (MQ=255) gTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGcaca < 1:761923/109‑1 (MQ=255) gTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGcaca > 2:761923/1‑109 (MQ=255) gTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACt > 1:310092/1‑138 (MQ=255) cATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGa > 1:202460/1‑139 (MQ=255) cATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCtgaatgaa < 2:500714/129‑1 (MQ=255) cATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCtgaatgaa > 1:500714/1‑129 (MQ=255) tAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGcaca < 2:833010/100‑1 (MQ=255) tAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGcaca > 1:833010/1‑100 (MQ=255) tAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACt > 1:660083/1‑139 (MQ=255) tAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACt > 2:547777/1‑139 (MQ=255) tAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACt > 1:348719/1‑139 (MQ=255) tAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACt > 1:497817/1‑139 (MQ=255) aaCAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCt < 2:438758/93‑1 (MQ=255) aaCAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCt > 1:438758/1‑93 (MQ=255) gCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGa < 2:473969/139‑1 (MQ=255) cGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCtgaatgaa < 1:500485/120‑1 (MQ=255) cGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCtgaatgaa > 2:500485/1‑120 (MQ=255) cGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGa > 2:732779/1‑138 (MQ=255) cGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGa > 1:230807/1‑138 (MQ=255) cGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGcc > 2:548370/1‑70 (MQ=255) cGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGcc < 1:548370/70‑1 (MQ=255) gTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTgcgc < 1:114015/63‑1 (MQ=255) gTTGCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTgcgc > 2:114015/1‑63 (MQ=255) gCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATccc > 1:548059/1‑100 (MQ=255) gCCCACGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATccc < 2:548059/100‑1 (MQ=255) aCGGCGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCt < 2:633707/139‑1 (MQ=255) cGAGATTCAAGTAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCAt > 2:556862/1‑139 (MQ=255) tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc < 2:230807/139‑1 (MQ=255) tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc < 2:348719/139‑1 (MQ=255) tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc < 2:778760/139‑1 (MQ=255) tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc < 1:181757/139‑1 (MQ=255) tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc < 2:348199/139‑1 (MQ=255) tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc < 2:645289/139‑1 (MQ=255) tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc < 2:310092/139‑1 (MQ=255) tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc < 2:202460/139‑1 (MQ=255) tAGCGGCGGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc < 1:646555/139‑1 (MQ=255) gcggcgGAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGCTa < 1:556862/139‑1 (MQ=255) cggcggAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCtt > 1:495002/1‑45 (MQ=255) cggcggAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCtt < 2:495002/45‑1 (MQ=255) cggcggAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc < 1:806346/136‑1 (MQ=255) cggcggAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGc > 2:806346/1‑136 (MQ=255) ggcggAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCt < 2:13129/120‑1 (MQ=255) ggcggAGCGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCt > 1:13129/1‑120 (MQ=255) | GTCGGCCCCAGGTAACGACCATTGATTCCCCAGACCGACGCGCGTGTCCCTGGCGTAAATGACCAGTGCGCACGTTGTACAGTCATAAACAGCGGTTGCCCACGGCGAGATTCAAGTAGCGGCGGAACGGGTAGCGGTTGCTGTTGCCCGGCTGCGCTGGCCTTCAGGGGAACAGCGCCTGCACAAAGTGCAATCCCCGATGCCTGAATGAACTGACGCCGACTGAGTGACATATTTGCTCCATGTAAAACTGGCTA > NZ_CP009273/3155796‑3156052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |