Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,193,900	A→G	L142P (CTC→CCC)	ygiF ←	inorganic triphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,193,900	0	A	G	100.0%	103.1 / NA	30	L142P (CTC→CCC)	ygiF	inorganic triphosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/15);  total (15/15)

ATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCGGTAA  >  NZ_CP009273/3193775‑3194033                                                                                                                              |                                                                                                                                      aTCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAAt                                                                                                                          >  2:120599/1‑139 (MQ=255) aTCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAAt                                                                                                                          >  2:216291/1‑139 (MQ=255)      gTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGc                                                                                                                     >  2:147899/1‑139 (MQ=255)                       gTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGccac                                                                                                     <  1:187940/138‑1 (MQ=255)                           gCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGc                                                                                                 >  1:852432/1‑138 (MQ=255)                                          gcgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaa                                                                                  >  2:719448/1‑138 (MQ=255)                                           cgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaaa                                                                                 >  2:469507/1‑138 (MQ=255)                                           cgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaaa                                                                                 >  2:227973/1‑138 (MQ=255)                                           cgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaaa                                                                                 >  1:763445/1‑138 (MQ=255)                                           cgcgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATaaa                                                                                 >  1:815180/1‑138 (MQ=255)                                             cgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                                                                              <  1:68519/139‑1 (MQ=255)                                             cgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                                                                              >  1:795707/1‑139 (MQ=255)                                             cgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                                                                              <  1:861214/139‑1 (MQ=255)                                             cgcgTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAAt                                                                              <  1:186155/139‑1 (MQ=255)                                                   tCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTg                                                                         <  1:852626/138‑1 (MQ=255)                                                              cagttccagttcCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGc                                                             <  1:209388/139‑1 (MQ=255)                                                                gttccagttccaGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTg                                                           <  1:227973/139‑1 (MQ=255)                                                                      gttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCAcgcg                                                     >  2:91038/1‑139 (MQ=255)                                                                      gttccagCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCAcgcg                                                     <  1:91038/139‑1 (MQ=255)                                                                                            aGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCA‑TTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAAtt                              >  2:44181/1‑139 (MQ=255)                                                                                                  ttCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCg                         <  2:191792/139‑1 (MQ=255)                                                                                                   tCACCCGCTTTCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGt                        >  2:429604/1‑139 (MQ=255)                                                                                                             tCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGa               <  2:763445/138‑1 (MQ=255)                                                                                                             tCACTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGa               <  2:815180/138‑1 (MQ=255)                                                                                                               aCTTCCCCCTGGTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACtt            <  2:852432/139‑1 (MQ=255)                                                                                                                         ggTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCGGTaa  >  1:36566/1‑139 (MQ=255)                                                                                                                         ggTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCGGTaa  >  2:777158/1‑139 (MQ=255)                                                                                                                          gTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCGGTaa  <  1:429604/138‑1 (MQ=255)                                                                                                                          gTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCGGTaa  <  1:235616/138‑1 (MQ=255)                                                                                                                          gTCGGGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCGGTa   <  1:216291/137‑1 (MQ=255)                                                                                                                              |                                                                                                                                      ATCCGGTTTGCGATACCAGTTGGTTCGCCAGTTTCAGCACCGCGCGCGTGTCGCCGCTAAGCAGTTCCAGTTCCAGCTCACAGATAGGTTCAGCAAATTCACCCGCTTTCACTTCCCCCTGGTCGAGGGCGATTTCAATTTGGCTACCATCGACCGCCACCAGCCATTTTTCGCGATAAAAATCGGTGCTGAACAGCGGCTGCACGCGGGAGGCGAGATCGGCGGGCAATTCGCCGTTCGGCCAGACTTCCGTCGGTAA  >  NZ_CP009273/3193775‑3194033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑