Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,202,275	T→G	V298G (GTG→GGG)	ttdT →	L‑tartrate/succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,202,275	0	T	G	100.0%	173.8 / NA	48	V298G (GTG→GGG)	ttdT	L‑tartrate/succinate antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/22);  total (26/22)

TACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCT  >  NZ_CP009273/3202154‑3202409                                                                                                                           |                                                                                                                                       tccccGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTcggcg                                                                                                                       <  1:757675/137‑1 (MQ=255) tACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTcggcg                                                                                                                       <  2:655330/139‑1 (MQ=255) tACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTcggcg                                                                                                                       >  1:678108/1‑139 (MQ=255) tACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTcggc                                                                                                                        <  1:494386/138‑1 (MQ=255)        cgGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATta                                                                                                                >  1:318376/1‑139 (MQ=255)                     tGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTcggcgg                                                                                                                      <  1:247515/120‑1 (MQ=255)                     tGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTcggcgg                                                                                                                      >  2:247515/1‑120 (MQ=255)                     tGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCcgc                                                                                                    >  1:631606/1‑138 (MQ=255)                       gCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGa                                                                                                  <  2:678108/138‑1 (MQ=255)                        cGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATg                                                                                                <  2:631606/139‑1 (MQ=255)                            cAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCg                                                                                            >  1:188718/1‑139 (MQ=255)                             aGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCgg                                                                                           >  2:406134/1‑139 (MQ=255)                             aGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCgg                                                                                           >  1:779436/1‑139 (MQ=255)                                     cgcTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCg                                                                                   >  2:677869/1‑139 (MQ=255)                                          ggCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCgtgg                                                                                <  2:652517/137‑1 (MQ=255)                                          ggCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCgtgg                                                                                <  1:122247/137‑1 (MQ=255)                                               agaCGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACt                                                                         >  1:790420/1‑139 (MQ=255)                                                    gAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACCGCGTGGTGGCACTGAtgc                                                                    >  2:220681/1‑139 (MQ=255)                                                    gAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGAtgc                                                                    >  2:788344/1‑139 (MQ=255)                                                    gAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGAtgc                                                                    <  2:513454/139‑1 (MQ=255)                                                    gAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGAtgc                                                                    >  1:40835/1‑139 (MQ=255)                                                     aaCTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGAtgct                                                                   <  1:788344/139‑1 (MQ=255)                                                          cAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCa                                                                           <  2:157387/126‑1 (MQ=255)                                                          cAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCa                                                                           >  1:157387/1‑126 (MQ=255)                                                          cAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTc                                                              >  2:703169/1‑139 (MQ=255)                                                           aGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCt                                                             >  1:54196/1‑139 (MQ=255)                                                                         ctctGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATg                                                                                                >  2:149383/1‑90 (MQ=255)                                                                         ctctGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATg                                                                                                <  1:149383/90‑1 (MQ=255)                                                                              ttCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGa                                                                                                  >  2:252775/1‑83 (MQ=255)                                                                              ttCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGa                                                                                                  <  1:252775/83‑1 (MQ=255)                                                                              ttCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGgac                                          >  2:41755/1‑139 (MQ=255)                                                                                   gTGAAAATCGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCa                                                                           <  1:277963/101‑1 (MQ=255)                                                                                   gTGAAAATCGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCa                                                                           >  2:277963/1‑101 (MQ=255)                                                                                    tGAAAAGCGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTGCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACAtt                                    <  1:283005/139‑1 (MQ=255)                                                                                      aaaaaCGGATGCTGGGGCTGATGGTAGGGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACAtt                                    <  1:180973/138‑1 (MQ=255)                                                                                      aaaaaCGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTcggcg                                                                                                                       <  1:634438/54‑1 (MQ=255)                                                                                      aaaaaCGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTcggcg                                                                                                                       >  2:634438/1‑54 (MQ=255)                                                                                                   ggggCTGATGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAgcg                     >  2:157971/1‑139 (MQ=255)                                                                                                          aTGGTA‑GGCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGa              <  1:546104/139‑1 (MQ=255)                                                                                                                 ggCGCGCTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGtt         <  2:392379/138‑1 (MQ=255)                                                                                                                     cgcTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTc                                 >  2:98248/1‑110 (MQ=255)                                                                                                                     cgcTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTc                                 <  1:98248/110‑1 (MQ=255)                                                                                                                       cTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCcg                                                                                                     >  2:66179/1‑40 (MQ=255)                                                                                                                       cTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCcg                                                                                                     <  1:66179/40‑1 (MQ=255)                                                                                                                       cTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGt                                  <  2:579068/107‑1 (MQ=255)                                                                                                                       cTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGt                                  >  1:579068/1‑107 (MQ=255)                                                                                                                       cTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTtcttct  >  2:554619/1‑139 (MQ=255)                                                                                                                       cTGGGGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTtcttct  >  1:812165/1‑139 (MQ=255)                                                                                                                           |                                                                                                                                       TACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGCCCGCTCTGTTCGCGTGAAAAACGGATGCTGGGGCTGATGGTA‑GGCGCGCTGGTGCTGTGGATTTTCGGCGGTGATTATATCGATGCCGCGATGGTCGGTTACAGCGTGGTGGCACTGATGCTGCTTCTGCGCATTATCAGTTGGGACGACATTGTCAGTAATAAAGCGGCGTGGAACGTTTTCTTCT  >  NZ_CP009273/3202154‑3202409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑