Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,210,830	A→C	intergenic (+69/+85)	nfeR → / ← aer	DNA‑binding transcriptional regulator NfeR/aerotaxis sensor receptor Aer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,210,830	0	A	C	100.0%	137.4 / NA	39	intergenic (+69/+85)	nfeR/aer	DNA‑binding transcriptional regulator NfeR/aerotaxis sensor receptor Aer
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/18);  total (21/18)

TGCACAAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTT  >  NZ_CP009273/3210695‑3210962                                                                                                                                        |                                                                                                                                     tGCAAAAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACcccc                                                                                                                                   <  2:564974/139‑1 (MQ=255)  gCACAAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCg                                                                                                                                  >  2:798318/1‑139 (MQ=255)   cacaAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCg                                                                                                                                  <  1:377472/138‑1 (MQ=255)   cacaAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGc                                                                                                                                 >  1:113429/1‑139 (MQ=255)   cacaAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGc                                                                                                                                 >  1:98266/1‑139 (MQ=255)      aaaTAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTa                                                                                                                               >  1:675141/1‑138 (MQ=255)        aTAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTaca                                                                                                                             >  1:152867/1‑138 (MQ=255)               aGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGc                                                                                                                     <  2:113429/139‑1 (MQ=255)                 aTCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAg                                                                                                                   <  2:434856/139‑1 (MQ=255)                                  ccGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGcc                                                                                                  >  2:525467/1‑139 (MQ=255)                                     cgccgcTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAg                                                                                                >  2:368341/1‑138 (MQ=255)                                     cgccgcTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAg                                                                                                >  2:266594/1‑138 (MQ=255)                                     cgccgcTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAg                                                                                                >  1:708090/1‑138 (MQ=255)                                       ccgcTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAgcat                                                                                             >  1:815106/1‑139 (MQ=255)                                       ccgcTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAgcat                                                                                             <  2:152867/139‑1 (MQ=255)                                                     aCGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCAtt                                                                                <  2:166175/138‑1 (MQ=255)                                                     aCGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATtg                                                                               >  1:434295/1‑139 (MQ=255)                                                       gCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATtgtg                                                                             <  2:742199/139‑1 (MQ=255)                                                           cgggtttAATCCCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTcc                                                                         <  2:708090/134‑1 (MQ=255)                                                           cTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTc                                                                          >  1:298083/1‑138 (MQ=255)                                                                        cgcATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGc                                                                                                                 <  2:843808/86‑1 (MQ=255)                                                                        cgcATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGc                                                                                                                 >  1:843808/1‑86 (MQ=255)                                                                        cgcATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATcc                                                            >  1:247500/1‑139 (MQ=255)                                                                              cGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATa                                                      <  1:266594/139‑1 (MQ=255)                                                                                   gTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGAt                                                 <  1:496539/139‑1 (MQ=255)                                                                                   gTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGAt                                                 <  1:236068/139‑1 (MQ=255)                                                                                   gTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGAt                                                 <  1:365366/139‑1 (MQ=255)                                                                                   gTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGAt                                                 <  1:343634/139‑1 (MQ=255)                                                                                       cgcgGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAAt                                             >  1:563080/1‑139 (MQ=255)                                                                                       cgcgGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAAt                                             >  2:44514/1‑139 (MQ=255)                                                                                                    ccGGATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCa                                 <  2:39266/138‑1 (MQ=255)                                                                                                     cGGATGCGACGCTTGACGCGCCTTACCCGGCCTACCCCCGCTACACCCCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCAcc                               <  2:172077/139‑1 (MQ=255)                                                                                                      ggATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACcg                              >  1:820149/1‑139 (MQ=255)                                                                                                      ggATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACcg                              >  1:206305/1‑139 (MQ=255)                                                                                                      ggATGCGACGCTTGACGCGCCTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACcg                              >  2:640710/1‑139 (MQ=255)                                                                                                                         ccTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGAt                                                 <  1:114583/101‑1 (MQ=255)                                                                                                                         ccTTATCCGGCCTACCCCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGAt                                                 >  2:114583/1‑101 (MQ=255)                                                                                                                             acccGCCCTCCCCCCGCTCCCCCCCCCGCAGGCCTGTTAGTATGCGCCAGCATCTCTTCAGGCTTTGTCCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGgcgc         <  2:432289/135‑1 (MQ=255)                                                                                                                             aTCCGGCCTCCCCCAGCTACACCCCCCGCTGGCCTGATAAGATGCGCCAGCTTCGCATCAGGCATTGTGCTCAACCCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGgcgc         <  2:298083/137‑1 (MQ=255)                                                                                                                                  gCCTACCCCCGCTACACACCCCGCAGGCCTGATAATATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGCTCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGttt  >  2:396763/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     TGCACAAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCCGCATCCGCCAGTGGCGCGGTGCAATTGCCGGATGCGACGCTTGACGCGCCTTATCCGGCCTACACCCGCTACACACCCCGCAGGCCTGATAAGATGCGCCAGCATCGCATCAGGCATTGTGCTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTT  >  NZ_CP009273/3210695‑3210962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑