Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,315,373	T→C	intergenic (‑401/+58)	yhbX ← / ← BW25113_RS16455	phosphoethanolamine transferase/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,315,373	0	T	C	100.0%	148.4 / NA	42	intergenic (‑401/+58)	yhbX/BW25113_RS16455	phosphoethanolamine transferase/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/22);  total (20/22)

CCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACC  >  NZ_CP009273/3315259‑3315500                                                                                                                   |                                                                                                                                ccAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCt                                                                                                                              <  1:602946/118‑1 (MQ=255) ccAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCt                                                                                                                              >  2:602946/1‑118 (MQ=255)  cAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGaaa                                                                                                         >  2:357302/1‑138 (MQ=255)  cAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGaaa                                                                                                         >  2:478982/1‑138 (MQ=255)        tGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGAc                                                                                                   <  1:431733/138‑1 (MQ=255)         gTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAg                                                                                                     >  1:839591/1‑135 (MQ=255)         gTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAg                                                                                                     <  2:839591/135‑1 (MQ=255)         gTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGc                                                                                                 >  2:114218/1‑139 (MQ=255)            aGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCtt                                                                                               <  1:531788/138‑1 (MQ=255)             gTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCtttt                                                                                             >  2:356814/1‑139 (MQ=255)                                    aaGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTg                                                                      >  1:337096/1‑139 (MQ=255)                                     aGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTgg                                                                     >  2:159099/1‑139 (MQ=255)                                     aGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTg                                                                      >  2:367850/1‑138 (MQ=255)                                          tACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTAcc                                                                 >  2:10232/1‑138 (MQ=255)                                            ccGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAg                                                              >  1:548567/1‑139 (MQ=255)                                                          tCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGaa                                                 <  2:184948/138‑1 (MQ=255)                                                            aaGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAAccc                                              <  1:367850/139‑1 (MQ=255)                                                            aaGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAAccc                                              <  1:159099/139‑1 (MQ=255)                                                            aaGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAAccc                                              <  1:10232/139‑1 (MQ=255)                                                              ggTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGt                                            <  2:337096/139‑1 (MQ=255)                                                                  ccAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAg                                         <  1:596132/138‑1 (MQ=255)                                                                   cAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGcc                                       >  1:219021/1‑139 (MQ=255)                                                                      cTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTa                                    <  2:493074/139‑1 (MQ=255)                                                                      cTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTa                                    <  1:350719/139‑1 (MQ=255)                                                                          gAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTgg                                >  1:826965/1‑139 (MQ=255)                                                                          gAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTgg                                >  2:814534/1‑139 (MQ=255)                                                                               aaGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCAc                            <  1:721173/138‑1 (MQ=255)                                                                                 gCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                         <  2:857782/139‑1 (MQ=255)                                                                                 gCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                         <  1:828468/139‑1 (MQ=255)                                                                                     cTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGaaaaa                                                                                                       >  2:570301/1‑57 (MQ=255)                                                                                     cTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGaaaaa                                                                                                       <  1:570301/57‑1 (MQ=255)                                                                                     cTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccacc                     <  1:572209/139‑1 (MQ=255)                                                                                       ggACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                         <  2:221713/133‑1 (MQ=255)                                                                                       ggACGAGATACAGATACCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                         >  1:221713/1‑133 (MQ=255)                                                                                            agatacagatacCTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGt              <  1:814534/139‑1 (MQ=255)                                                                                                       ccTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc   >  2:493206/1‑139 (MQ=255)                                                                                                       ccTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAc   >  2:805156/1‑139 (MQ=255)                                                                                                        cTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAcc  <  1:356814/139‑1 (MQ=255)                                                                                                        cTGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAcc  <  1:114218/139‑1 (MQ=255)                                                                                                         tGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                         <  1:721645/115‑1 (MQ=255)                                                                                                         tGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTac                         >  2:721645/1‑115 (MQ=255)                                                                                                         tGACAACCATCCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTAcc  >  2:843731/1‑138 (MQ=255)                                                                                                                   |                                                                                                                                CCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACC  >  NZ_CP009273/3315259‑3315500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑