Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,326,882	A→G	intergenic (‑72/‑187)	rplU ← / → ispB	50S ribosomal protein L21/octaprenyl diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,326,882	0	A	G	100.0%	200.4 / NA	55	intergenic (‑72/‑187)	rplU/ispB	50S ribosomal protein L21/octaprenyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/27);  total (28/27)

CGCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCT  >  NZ_CP009273/3326765‑3327015                                                                                                                      |                                                                                                                                      cGCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGcc                                                                                                                  >  2:829968/1‑139 (MQ=255)      aCTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGa                                                                                                             >  2:598827/1‑139 (MQ=255)       cTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGAc                                                                                                            <  2:663968/139‑1 (MQ=255)          ggTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACaa                                                                                                          <  2:584055/138‑1 (MQ=255)              ttgttTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCt                                                                                                     <  1:91908/139‑1 (MQ=255)                 tttACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTAc                                                                                                   <  2:126324/138‑1 (MQ=255)                   tACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAg                                                                                                 <  1:598827/138‑1 (MQ=255)                    accaccACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                      >  2:681016/1‑116 (MQ=255)                    accaccACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                      <  1:681016/116‑1 (MQ=255)                      caccacTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTa                                                                                                    <  1:742503/132‑1 (MQ=255)                      caccacTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTa                                                                                                    >  2:742503/1‑132 (MQ=255)                      caccacTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCaa                                                                                             >  2:647646/1‑139 (MQ=255)                      caccacTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCaa                                                                                             >  1:757030/1‑139 (MQ=255)                      caccacTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCaa                                                                                             >  2:727064/1‑139 (MQ=255)                           cTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                      >  2:576658/1‑109 (MQ=255)                           cTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                      <  1:576658/109‑1 (MQ=255)                           cTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATacac                                                                                        >  1:622630/1‑139 (MQ=255)                           cTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATacac                                                                                        >  2:502001/1‑139 (MQ=255)                              tGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGAc                                                                                                            >  1:446194/1‑116 (MQ=255)                              tGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGAc                                                                                                            <  2:446194/116‑1 (MQ=255)                                                       ccGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACga                                                                                      >  2:90882/1‑113 (MQ=255)                                                       ccGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACga                                                                                      <  1:90882/113‑1 (MQ=255)                                                        cGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaaaaaa                                                                            <  2:160017/122‑1 (MQ=255)                                                        cGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaaaaaa                                                                            >  1:160017/1‑122 (MQ=255)                                                        cGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACgg                                                              >  2:199398/1‑136 (MQ=255)                                                        cGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACgg                                                              <  1:199398/136‑1 (MQ=255)                                                        cGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGt                                                             <  2:622630/137‑1 (MQ=255)                                                        cGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGt                                                             <  2:864308/137‑1 (MQ=255)                                                                     acCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGc                                              >  2:212441/1‑139 (MQ=255)                                                                            cAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAtttt                                        <  1:720303/138‑1 (MQ=255)                                                                                 aTTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                      >  1:565258/1‑55 (MQ=255)                                                                                 aTTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAacgca                                                                                                                      <  2:565258/55‑1 (MQ=255)                                                                                 aTTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTa                                                                                                    >  2:207321/1‑73 (MQ=255)                                                                                 aTTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTa                                                                                                    <  1:207321/73‑1 (MQ=255)                                                                                       agTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATc                            <  1:212441/139‑1 (MQ=255)                                                                                        gTGCGCTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCa                           <  2:53797/139‑1 (MQ=255)                                                                                           cgcTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCATGCCTTTGAc                                                                                                            <  1:50986/55‑1 (MQ=255)                                                                                           cgcTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCATGCCTTTGAc                                                                                                            >  2:50986/1‑55 (MQ=255)                                                                                           cgcTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAccc                        >  1:178410/1‑139 (MQ=255)                                                                                           cgcTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAcc                         <  1:502001/138‑1 (MQ=255)                                                                                             cTATAAATATTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCta                      >  1:759823/1‑139 (MQ=255)                                                                                                     atTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACtt                                                                    <  1:820813/85‑1 (MQ=255)                                                                                                     atTCACAATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACtt                                                                    >  2:820813/1‑85 (MQ=255)                                                                                                        cacaATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAAc            >  2:192858/1‑138 (MQ=255)                                                                                                        cacaATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAAc            >  2:175938/1‑138 (MQ=255)                                                                                                          caATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCa                           >  1:749723/1‑121 (MQ=255)                                                                                                          caATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCa                           <  2:749723/121‑1 (MQ=255)                                                                                                          caATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCAt         <  2:178410/139‑1 (MQ=255)                                                                                                          caATAGGGCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCAt         <  2:759823/139‑1 (MQ=255)                                                                                                                ggCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCt                                                                                                     <  2:548577/41‑1 (MQ=255)                                                                                                                ggCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCt                                                                                                     >  1:548577/1‑41 (MQ=255)                                                                                                                ggCGCGGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAAccc   >  1:574326/1‑139 (MQ=255)                                                                                                                  cgcgGATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCt  >  1:25483/1‑138 (MQ=255)                                                                                                                   gcgGATATTACGCAAAACGCACGCCTTTGACAAGTGCTAc                                                                                                   <  2:711031/40‑1 (MQ=255)                                                                                                                   gcgGATATTACGCAAAACGCACGCCTTTGACAAGTGCTAc                                                                                                   >  1:711031/1‑40 (MQ=255)                                                                                                                      |                                                                                                                                      CGCTTACTCGGTGTTGTTTACCACCACTTTGGAAAACCGCGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCT  >  NZ_CP009273/3326765‑3327015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑